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| Cloning of HD-Zip Transcription Factor Pehox14 Gene in Moso Bamboo (Phyllostachys edulis) and Its Functional Analysis Under Drought and Salt Stress |
| LIU Yu-Jiao, YAN Xiao-Ling, CHANG Xin, XIAO Ya-Qian, CAO Shan, GUO Xiao-Qin* |
| National Key Laboratory for Development and Utilization of Forest Food Resources/Key Laboratory of Bamboo Science and Technology of Ministry of Education/Bamboo Industry Institute, Zhejiang A&F University, Hangzhou 311300, China |
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Abstract The homeodomain-leucine zipper (HD-Zip) protein is a specific transcription factor in plants, playing important regulatory roles in plant growth, development and environmental stress response. Moso bamboo (Phyllostachys edulis) grows rapidly and exhibits strong adaptability. It can cope with environmental stress and maintain normal growth and development under abiotic stress. In order to explore the expression pattern of the HD-Zip gene in moso bamboo under drought and salt stress, this study obtained the HD-Zip family member Pehox14 (GenBank No. PQ806999) gene from moso bamboo. Sequence analysis showed that the ORF length of the Pehox14 gene was 699 bp, encoding 232 amino acids, including 3 exons and 2 introns. The Pehox14 protein had a conserved Homeodomain domain and a special HALZ (Leucine Zipper) domain. Subcellular localization showed that Pehox14 was located in nucleus. By using qRT-PCR to analyze the expression patterns under drought and salt stress conditions, the results showed that the expression of Pehox14 gene was significantly upregulated under drought and salt stress. Transgenic Arabidopsis plants were generated by Agrobacterium infection. Analysis of abiotic stress indicated that when transgenic Arabidopsis plants were subjected to mannitol and NaCl treatment, their germination rate, survival rate and plant height were significantly higher than those of control, indicating that overexpression of Pehox14 could enhance the tolerance of transgenic Arabidopsis to drought and salt stress. In addition, under stress conditions of 200 mmol/L mannitol and 200 mmol/L NaCl, the expression of stress-related genes, such as AtRD29A (responsive to desiccation 29A), AtRD22 (responsive to desiccation 22), AtLEA (late embryogenesis abundant), AtSOS1 (salt overly sensitive 1), AtNHX1 (Na+/H+ exchanger 1) and AtHKT (high-affinity K+ transporter) was significantly upregulated in transgenic plants. Overall, Pehox14 plays a positive regulatory role in response to drought and salt stress. This study provides a theoretical basis for investigating the molecular regulation mechanism of HD-Zip transcription factors in stress resistance of moso bamboo.
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Received: 25 October 2024
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Corresponding Authors:
*xqguo@Zafu.edu.cn
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