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| Identification of Differentially Expressed Transcription Factors in Sesame (Sesamum indicum) in Response to High Temperature and Expression Characteristics Analysis of SiMYB4 Gene |
| WANG Dong-Yong1,2, ZHANG Peng-Yu1,2, LI Feng1,2, TIAN Yuan1,2, RONG Ya-Si1, LU Hai-Ling1, WAN Fu-Qiang3, GAO Tong-Mei1,2,* |
1 Sesame Research Center/Henan Key Laboratory of Specific Oilseed Crops Genomics, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
2 The Shennong Laboratory, Zhengzhou 450002, China;
3 Pingyu County Agriculture and Rural Affairs Bureau, Zhumadian 463400, China |
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Abstract High temperature is an important factor that restricts the healthy and sustainable development of sesame (Sesamum indicum) production. The aim of this study is to reveal the molecular mechanism and mining key transcription factors in response to high temperature stress of sesame, and analyze the expression characteristics of candidate genes under different stresses. In this study, the leaves of heat-tolerant sesame variety 'Zhengtaizhi No.3' and heat-sensitive sesame variety 'Shandong White Sesame' were collected after 10 d of high temperature treatment for transcriptome sequencing. The results showed that 954 transcription factors were identified in 2 sesame varieties under high temperature treatment, and 255 transcription factors were differentially expressed, accounting for 26.73% of the total. The most differentially expressed transcription factor families were bHLH (basic helix-loop-helix), AP2/ERF (ethylene responsive factor), NAC (NAM, ATAF1/2, CUC1/2), MYB (avian myeloblastosis viral oncogenehomolog) and C2H2 families. Compared with CK, 191 and 206 differentially expressed transcription factors were detected in 'Shandong white sesame' and 'Zhengtaizhi No.3' under high temperature treatment, respectively, among which 50 and 65 transcription factors were specifically expressed in 'Shandong White Sesame' and 'Zhengtaizhi No.3', respectively. The Venn diagram showed that a total of 141 transcription factors were differentially expressed in 2 varieties, of which 38 transcription factors were up-regulated and 103 transcription factors were down-regulated. In addition, 36 thermotolerant transcription factors were identified, among which MYB family had the largest number of transcription factors. Six key candidate genes in response to high temperature stress, such as SiMYB4, SibZIP41 and SiHsfB2b were screened by co-expression network analysis. The promoter region of the thermotolerant SiMYB4 contains multiple hormone and stress response elements. qRT-PCR results showed that SiMYB4 was induced to express under high temperature, drought, high salinity and ABA treatments. The expression of SiMYB4 reached the maximum at 48 h of high temperature and ABA treatment, which were 10.28 times and 10.80 times that of 0 h respectively, and the differences were significant. Under drought, high salt and MeJA treatments, the expression of SiMYB4 gene increased first and then decreased. This study provides theoretical basis and candidate genes for further exploring the molecular mechanism heat resistance in sesame.
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Received: 05 December 2024
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Corresponding Authors:
*gaotongmei@126.com
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