IGF-1、IGF-2和TGF-β2在不同年龄牦牛肺脏中的表达分析

doi:10.3969/j.issn.1674-7968.2025.06.009

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (13229 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Insulin like growth factor (IGF) IGF-1 and IGF-2, as hypoxia target factors, can cooperate with transforming growth factor- β2 (TGF- β2) to participate in lung growth and development, immune regulation and injury repair. The purpose of this study was to investigate the expression and distribution characteristics of IGF-1, IGF-2 and TGF- β2 in the lungs of yaks (Bos grunniens) in different ages. The distribution and expression levels of IGF-1, IGF-2 and TGF- β2 in the lungs of yaks at different ages were investigated by immunohistochemical staining, qRT-PCR and Western blot. Immunohistochemical analysis showed that the expression positions of the three growth factors in the yak lungs were similar, and they were mainly distributed in all levels of bronchial epithelial cells and alveolar epithelial cells. In addition, IGF-1 and IGF-2were also expressed in pulmonary artery endothelial cells. The results of qRT-PCR showed that the expression levels of IGF-1 and TGF-β2 genes in the lungs of adult yaks were significantly higher than those of newborn, juvenile and elderly yaks (P<0.05). The expression of IGF-2 gene in the lung of newborn yak was significantly higher than that in other age groups (P<0.05). The results of Western blot showed that theexpression of IGF-1 and IGF-2 protein was the highest in the primary stage and significantly higher than that in other age groups (P<0.05). The expression of TGF- β2 protein was the highest in adulthood, and was significantly higher than that in other age groups (P<0.05). The expression levels of the 3 factors were similar with age, and the expression levels were higher in newborn and adulthood, suggesting that IGF-1, IGF-2, and TGF-β2 might be involved in the development of lung and the formation of hypoxic adaptive structure in yak at this age. This study provides the basis for exploring the specific adaptation mechanism of yak lung in hypoxic environment.

Key words： Yak Lung Insulin like growth factor 1 (IGF-1) IGF-2 Transforming growth factor-β2 (TGF-β2)

Received: 13 August 2024

ZTFLH:

S823.85

Corresponding Authors: *cuiyan369@sina.com

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	DENG Yan-Jiang
	ZHONG Dong-Lan
	CUI Yan
	YU Si-Jiu
	WEI Peng-Qiang
	ZHANG Qian
	NIU Yue-Yue
	XIAN Kang-Le
	WANG Deng-Hui
	CHEN Chun-Yan

Cite this article:

DENG Yan-Jiang,ZHONG Dong-Lan,CUI Yan, et al. Expression Analysis of IGF-1, IGF-2 and TGF- β2 in the Lung of Yaks(Bos grunniens) in Different Ages[J]. 农业生物技术学报, 2025, 33(6): 1280-1289.

URL:

https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.06.009 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I6/1280

[1] 陈学群, 王世君, 刘宇, 等. 2007. IGF 家族与低氧损伤和适应性[C]//中国生理学会 2007 年消化内分泌生殖学术研讨会论文摘要汇编 , pp. 8-9.
(Chen X Q, Wang S J, Liu Y, et al.2007. The IGF family and hypoxic damage and adaptation[C]. Chinese Physiological Society 2007 Digestive Endocrinology Reproductive Academic Sym-posium Paper Abstract Compilation, pp. 8-9.)
[2] 贡继尚.2017. IGF2 基因对牦牛和小鼠成纤维细胞的增殖及相关信号的研究[D]. 博士学位论文, 甘肃农业大学, 导师 , 赵兴绪 , pp. 7.
(Gong J S. 2017. Study on IGF2 gene proliferation and related signaling in yak and mouse fibroblasts[D]. Thesis for Ph. D., Gansu Agricul-tural University, Supervisor: Zhao X X, pp. 7.)
[3] 郭思彤, 尚莉丽. 2022. 息风定喘方对哮喘豚鼠血清 VEGF、EGF、TGF-β2 的调节作用[J]. 中国医药导报 , 19(09): 27-30.
(Guo S T, Shang L L, 2022. Regulation of Xi-fengdingchuan decoction on serum VEGF, EGF and TGF-β2 in guinea pigs with asthma[J]. China Medical Herald, 19(09): 27-30.)
[4] 李娜, 李占强, 芦殿香. 2024. 靶向肺血管内皮功能障碍治疗肺动脉高压的研究进展[J]. 中国药理学通报, 40(01): 1-5.
(Li N, Li Z Q, Lu D X, et al.2024. Research prog-ress of targeted pulmonary vascular endotheli-al dys-function in treatment of pulmonary hypertension[J]. Journal of Chinese Pharmacology Bulletin, 40(01): 1-5.)
[5] 王可金, 文德全, 崔燕, 等. 2023. TGF-β1/Smad 信号通路相关因子在牦牛肺脏低氧适应中的表达分析[J]. 农业生物技术学报 , 31(01): 105-114.
(Wang K J, Wen D Q, Cui Y, et al.2023. Expression analysis of TGF-β1/Smad signaling pathway related factors during lung hypoxia adaptation in yak(Bos grunniens)[J]. Journal of Agricul-tural Biotechnology, 31(01): 105-114.)
[6] 翟国亮, 崔燕, 余四九, 等. 2024. HIF-2α、LP 及 LEPR 在不同年龄牦牛肺组织中的表达[J]. 中国兽医科学, 54(09): 1259-1266.
(Zhai G L, Cui Y, Yu S J, et al.2024. Ex-pression of HIF-2α, LP and LEPR in lung tissues of yaks at different ages[J]. Journal of Chinese Veterinary Science, 54(09): 1259-1266.)
[7] 张慧珠.2021. 基于多组学分析牦牛肺动脉平滑肌细胞低氧适应性机制的研究[D]. 博士学位论文, 甘肃农业大学, 导师 : 崔燕 , pp. 12-14.
(Zhang H Z.2021. Hypoxic adaptability of yak pulmonary artery smooth muscle cells based on multi-omics analysis[D]. Thesis for Ph. D., Gansu Agricultural University, Supervisor: Cui Y, pp.12-14.)
[8] 张月文, 王甜, 姜璐瑶, 等. 2024. 高原低氧环境对机体损伤的研究进展[J]. 联勤军事医学 , 38(09): 813-817.
(Zhang Y W, Wang T, Jiang L Y, et al.2024. Research progress on body damage caused by low oxygen envi-ronment at high altitude[J]. Military Medicine of Joint Logistics, 38(09): 813-817.
[9] 肇涛澜, 张硕, 钱文峰. 2020. 翻译延伸的顺式调控机理与生物学效应[J]. 遗传, 42(07): 613-631.
(Zhao T L, Zhang S, Qian W F.2020. Cis regulation mechanism and bio-logical effects of translation extension[J]. Heredity, 42(07): 613-631.)
[10] Akman H O, Zhang H, Siddiqui M A, et al.2001. Response to hypoxia involves transforming growth factor-beta2 and Smad proteins in human endothelial cells[J]. Blood, 98(12): 3324-3331.
[11] Ayalew W, Chu M, Liang C, Wu X, et al.2021. Adaptation mechanisms of yak(Bos grunniens)to high-altitude envi-ronmental stress[J]. Animals, 11(8): 2344.
[12] Bissonnette E Y, Lauzon-Joset J F, Debley J S, et al, 2020.Cross-talk between alveolar macrophages and lung epi-thelial cells is essential to maintain lung homeostasis[J]. Frontiers in Immunology, 11: 583042.
[13] Chao W, D'Amore P A.2008. IGF2: Epigenetic regulation and role in development and disease[J]. Cytokine & Growth Factor Reviews, 19(2): 111-120.
[14] Dallinga M G, Yetkin-Arik B, Kayser R P, et al.2018. IGF2 and IGF1R identified as novel tip cell genes in primary microvascular endothelial cell monolayers[J]. Angiogen-esis, 21(4): 823-836.
[15] Du L, Lin L, Li Q, et al.2019. IGF-2 Preprograms maturing macrophages to acquire oxidative phosphorylation-de-pendent anti-inflammatory properties[J]. Cell Metabo-lism, 29(6): 1363-1375.e8.
[16] Esteves P, Allard B, Celle A, et al.2022. Asthmatic bronchial smooth muscle increases rhinovirus replication within the bronchial epithelium[J]. Cell Reports, 38(13): 110571.
[17] Garrett S M, Hsu E, Thomas J M, et al.2019. Insulin-like growth factor(IGF)-II-mediated fibrosis in pathogenic lung conditions[J]. PLOS ONE, 14(11): e0225422.
[18] Gao F, Li C, Smith S M, Peinado N, et al.2022. Decoding the IGF1 signaling gene regulatory network behind alveolo-genesis from a mouse model of bronchopulmonary dys-plasia[J]. eLife, 11: e77522.
[19] Ge Q, Guo Y, Zheng W, et al.2021. Molecular mechanisms detected in yak lung tissue via transcriptome-wide analy-sis provide insights into adaptation to high altitudes[J]. Scientific Reports, 11(1): 7786.
[20] Halwani R, Al-Muhsen S, Al-Jahdali H, et al.2011. Role of transforming growth factor-β in airway remodeling in asthma[J]. American Journal of Respiratory Cell and Molecular Biology, 44(2): 127-133.
[21] Han C Z, Juncadella I J, Kinchen J M, et al.2016. Macro-phages redirect phagocytosis by non-professional phago-cytes and influence inflammation[J]. Nature, 539(7630): 570-574.
[22] He J, Mu M, Wang H, et al.2019. Upregulated IGF 1 in the lungs of asthmatic mice originates from alveolar macro-phages[J]. Molecular Medicine Reports, 19(2): 1266-1271.
[23] Ivy C M, Scott G R.2015. Control of breathing and the circu-lation in high-altitude mammals and birds[J]. Compara-tive Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 186: 66-74.
[24] Kim I, Kim C H, Yim Y S, et al.2008. Autocrine function of erythropoietin in IGF-1-induced erythropoietin biosyn-thesis[J]. NeuroReport, 19(17): 1699-1703.
[25] Ko H K, Hsiao Y H, Jeng M J, et al.2023. The role of trans-forming growth factor-β2 in cigarette smoke-induced lung inflammation and injury[J]. Life Sciences, 320:121539.
[26] Lallemand A V, Ruocco S M, Joly P M, et al.1995. In vivo lo-calization of the insulin-like growth factors I and II(IGFI and IGF II)gene expression during human lung devel-opment[J]. The International Journal of Developmental Biology, 39(3): 529-537.
[27] Li C, Li Y, He L, et al.2013a. PI3K/AKT signaling regulates bioenergetics in immortalized hepatocytes[J]. Free Radi-cal Biology & Medicine, 60: 29-40.
[28] Li J, Masood A, Yi M, et al.2013b. The IGF-I/IGF-R1 path-way regulates postnatal lung growth and is a nonspecif-ic regulator of alveologenesis in the neonatal rat[J]. American Journal of Physiology. Lung Cellular and Mo-lecular Physiology, 304(9): L626-L637.
[29] Liu H, Zhou J, Degen A, et al.2022. A comparison of average daily gain, apparent digestibilities, energy balance, ru-men fermentation parameters, and serum metabolites be-tween yaks(Bos grunniens)and Qaidam cattle(Bos tau-rus)consuming diets differing in energy level[J]. Ani-mal Nutrition, 12: 77-86.
[30] Liu J, Tseu I, Wang J, Tanswell K, et al.2000. Transforming growth factor beta2, but not beta1 and beta3, is critical for early rat lung branching[J]. Developmental Dynam-ics : An Official Publication of the American Associa-tion of Anatomists, 217(4): 343-360.
[31] Massagué J, Sheppard D.2023. TGF-β signaling in health and disease[J]. Cell, 186(19): 4007-4037.
[32] Mu M, Gao P, Yang Q, et al.2020. Alveolar epithelial cells promote IGF-1 production by alveolar macrophages through TGF-β to suppress endogenous inflammatory signals[J]. Frontiers in Immunology, 11: 1585.
[33] Narasaraju T A, Chen H, Weng T, et al.2006. Expression pro-file of IGF system during lung injury and recovery in rats exposed to hyperoxia: A possible role of IGF-1 in al-veolar epithelial cell proliferation and differentiation[J]. Journal of Cellular Biochemistry, 97(5): 984-998.
[34] Noveral J P, Bhala A, Hintz R L, et al.1994. Insulin-like growth factor axis in airway smooth muscle cells[J]. The American Journal of Physiology, 267(6 Pt 1): L761-L765.
[35] Otake S, Sasaki T, Shirai T, et al.2021. CLEC-2 stimulates IGF-1 secretion from podoplanin-positive stromal cells and positively regulates erythropoiesis in mice[J]. Jour-nal of Thrombosis and Haemostasis, 19(6): 1572-1584.
[36]Qi X, Zhang Q, He Y, et al. 2019. The transcriptomic land-scape of yaks reveals molecular pathways for high alti-tude adaptation[J]. Genome Biology and Evolution, 11(1): 72-85.
[37] Sall J W, Klisovic D D, O'Dorisio M S, et al.2004. Somatosta-tin inhibits IGF-1 mediated induction of VEGF in hu-man retinal pigment epithelial cells[J]. Experimental eye Research, 79(4): 465-476.
[38] Schwanhäusser B, Busse D, Li N, et al.2011. Global quantifi-cation of mammalian gene expression control[J]. Na-ture, 473(7347): 337-342.
[39] Sélénou C, Brioude F, Giabicani E, et al.2022. IGF2: Devel-opment, genetic and epigenetic abnormalities[J]. Cells,11(12): 1886.
[40] Senavirathna L K, Huang C, Yang X, et al.2018. Hypoxia in-duces pulmonary fibroblast proliferation through NFAT signaling[J]. Scientific Reports, 8(1): 2709.
[41] Sun T, Huang Z, Liang W C, et al.2021. TGFβ2 and TGFβ3 isoforms drive fibrotic disease pathogenesis[J]. Science Translational Medicine, 13(605): eabe0407.
[42] Sun Y, Liu W Z, Liu T, et al.2015. Signaling pathway of MAPK/ERK in cell proliferation, differentiation, migra-tion, senescence and apoptosis[J]. Journal of Receptor and Signal Transduction Research, 35(6): 600-604.
[43] Takahashi H, Alves C R R, Stanford K I, et al.2019. TGF-β2 is an exercise-induced adipokine that regulates glucose and fatty acid metabolism[J]. Nature Metabolism, 1(2): 291-303.
[44] Vogel C, Marcotte E M.2012. Insights into the regulation of protein abundance from proteomic and transcriptomic analyses[J]. Nature Reviews Genetics, 13(4): 227-232.
[45] Wang M Y, Liu W J, Wu L Y, et al.2023. The research prog-ress in transforming growth factor-β2[J]. Cells, 12(23): 2739.
[46] Xie Y, Shi X, Sheng K, et al.2019. PI3K/Akt signaling trans-duction pathway, erythropoiesis and glycolysis in hypox-ia(Review)[J]. Molecular Medicine Reports, 19(2): 783-791.