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| Functional Study of OsGASR6 Gene in Rice (Oryza sativa) |
| WANG Cheng1, LIU Tao1,2,* |
1 College of Life Sciences and Resources and Environment, YiChun University, Yichun 336000, China;
2 Key Laboratory of Crop Growth and Development Regulation in Jiangxi Province, Yichun 336000, China |
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Abstract The OsGASR6 (GA-stimulated transcript-related 6) gene of rice (Oryza sativa) pertains to the plant gibberellic acid-stimulated transcript (GAST) gene superfamily. Existing research has demonstrated that this gene family regulates the growth of plant cells and influences the morphology and size of plant organs. In this study, bioinformatics prediction and analysis of the sequence of the OsGASR6 gene disclosed that the OsGASR6 gene possessed typical sequence characteristics of GAST-like genes. The promoter region contained numerous cis-acting elements responsive to hormones, stress, light, and tissue-specific expression. The subcellular localization prediction and localization results of fusion expression vector showed that OsGASR6 was located in the extracellular space. The promoter activity of OsGASR6 gene was analyzed using the β-glucuronidase (GUS) reporter gene, and it was discovered that the OsGASR6 promoter was higher in rice endosperm, and it was also active in roots and stems. Phenotypic identification and analysis of rice lines with overexpression and RNAi interference of OsGASR6 gene expression were carried out. It was found that overexpression of the OsGASR6 gene extremely significantly reduced the height of rice stems (P<0.01), while the seed thickness of RNAi interference lines extremely significantly increased (P<0.01). This study provides a critical target for molecular breeding aimed at improving rice plant architecture and grain quality.
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Received: 22 August 2024
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Corresponding Authors:
*liutao0727@126.com
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