迷迭香酸对奶山羊精液冷冻保存作用的研究

doi:10.3969/j.issn.1674-7968.2025.04.011

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Abstract Sperm cells are highly susceptible to damage during semen cryopreservation, in which excessive free radicals cause oxidative stress that negatively affects the cellular system by increasing the production of reactive oxygen species. Therefore, the use of antioxidants in semen cryopreservation is crucial. Rosmarinic acid (RA) is a natural polyphenolic antioxidant with strong antioxidant activity. In this study, 0, 20, 40, 80 and 120 μmol/L RA was added to the semen cryopreservation diluent of dairy goats (Capra hircus) to test the sperm quality and sperm antioxidant capacity, and sperm plasma metabolic profiles of the spermatozoa of dairy goats were analyzed by LC-MS non-targeted metabolomics after freezing. The results showed that RA improved the quality of cryopreserved dairy goat spermatozoa and sperm antioxidant capacity, and 309 differential metabolites were detected between the control group and RA-treated group. Moreover, RA regulated the energy metabolism of spermatozoa through AMP-activated protein kinase (AMPK) signaling pathway, and activated or inhibited apoptosis-related proteins, such as B-cell lymphoma-2 (Bcl-2), through PI3K-Akt signaling pathway. The present study demonstrated that the addition of 40 μmol/L RA could improve the effect of semen cryopreservation in dairy goats. This study provides a new idea and theoretical basis for the optimization of semen cryopreservation technology in dairy goats.

Key words： Rosemarinic acid (RA) Semen cryopreservation Semen quality Antioxidant capacity Metabolomics technology

Received: 08 July 2024

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Corresponding Authors: * anxiaopengdky@163.com

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	ZHANG Pei
	XU Gang-Gang
	YUAN Yuan
	ZHANG Jian-Yong
	YAN Tian-Rong
	PENG Zhong-Hong
	LI Zong-Ru
	HOU Jin-Xing
	AN Xiao-Peng

Cite this article:

ZHANG Pei,XU Gang-Gang,YUAN Yuan, et al. Effect of Rosemarinic Acid on the Cryopreservation of Dairy Goats (Capra hircus) Semen[J]. 农业生物技术学报, 2025, 33(4): 823-836.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.04.011 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I4/823

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