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| Cloning and Tissue Expression Analysis of Coat Color Candidate Gene STX17 in Maiwa Yak (Bos grunniens) |
| SHI Lin1*, GUO Zi-Xuan1*, LI Xiao-Wei2, MA Shi-Long1, HE Xiao-Qiang2, JIANG Ming-Feng1**, LIU Yi-Li1** |
1 College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China;
2 Sichuan Longri Livestock Farm, Hongyuan 624400, China |
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Abstract Maiwa yak (Bos grunniens) is a local superior seed of Qinghai-Tibet Plateau yak. Previous studies have shown that the coat color of Maiwa yak is related to its production performance and can be used as the basis for breeding. Previous research has shown that the grey coat trait in Maiwa yak is associated with the expression level of the syntaxin-17 (STX17) gene. The STX17 protein is involved in the fusion process of lysosomes and autophagosomes, playing an important role in the synthesis, transport of melanin, and the development of melanoma. To investigate the correlation between the STX17 gene and coat color formation in Maiwa yak, 3 healthy adult male black and grey Maiwa yaks aged 3 years were selected in this study, and their heart, spleen, lung, kidney and skin tissues were selected as experimental materials. The CDS sequence of the STX17 gene were cloned by PCR, and bioinformatics analysis was carried out by using on line software, the expression of the STX17 gene was detected in different tissues and coat populations of Maiwa yak by qRT-PCR. The results showed that the CDS region of the STX17 gene in Maiwa yak was 909 bp, encodimg protein contained 302 amino acids with a theoretical value of isoelectric point (pI) of 5.82, which was an acidic protein, the STX17 protein did not have a signal peptide sequence, and there was 1 target membrane-associated soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) domain and 2 tandem transmembrane domains; Subcellular localisation predicted that the STX17 protein mainly existed in the cytoplasm. The nucleotide sequence comparison showed that the STX17 gene in Maiwa yak was most closely related to Bos taurus, followed by Bubalis bubalus. The STX17 protein was closely related to autophagy-related protein 14 (ATG14), synaptosome associated protein of 29 (SNAP29), vesicle associated membrane protein 7 (VAMP7), vesicle associated membrane protein 8 (VAMP8), synaptobrevin homolog (YKT6) and other proteins. The qRT-PCR results showed that the STX17 gene had the highest expression in skin tissues, and the expression in the skin of grey Maiwa yak was higher than black Maiwa yak, it was hypothesized that the STX17 gene was related to the formation of grey and black coat color in Maiwa yak. This study provides theoretical basis for the subsequent studies on the regulatory mechanism of coat color in Maiwa yak.
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Received: 26 July 2024
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Corresponding Authors:
** mingfengjiang@vip.sina.com;yililiunum@163.com
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| About author:: * These authors contributed equally to this work |
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