Screening, Construction and Optimization of Microbial Communities for Multifunctional Biocontrol of Tobacco Brown Spot
WU Xu-Yan1,2, LI Xing-Shan1,2, CUI Chuan-Bin4, WANG Ping-Ping4, HUANG Li-Li2,3,*, WANG Na-Na1,2,*
1 College of Life Sciences, Northwest A&F University, Yangling 712100, China; 2 National Key Laboratory of Crop Stress Resistance and Efficient Production, Yangling 712100, China; 3 College of Plant Protection, Northwest A&F University, Yangling 712100, China; 4 Shaanxi Provincial Company, China National Tobacco Corporation, Xi'an 710000, China
Abstract Tobacco brown spot is the main fungal disease that endangers the tobacco industry. Biological control is an important means to control plant diseases. Synthetic community has been a research hotspot in the field of biological control because of its good growth-promoting and stress-resistant functions in agricultural production.The rhizosphere soil of healthy tobacco (Nicotiana tabacum) plants in the tobacco field with severe brown spot disease in southern Shaanxi was used as the material, and the strains with significant antagonistic effect on Alternaria alternate were isolated and screened by dilution separation, plate confrontation method and mycelial growth rate method. The growth-promoting strains were screened by measuring the ability of bacteria to produce indole acetic acid (IAA) and growth-promoting pot experiments. The antibacterial strains were screened by measuring the activities of phenylalaninammo-nialyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) defense enzymes in tobacco leaves, and then the selected strains were identified by morphological, physiological and biochemical characteristics and molecular biological methods. Then, the Box-Benhnken response surface method was used to optimize the ratio of bacteria to finally construct a multi-functional biocontrol bacteria group. The screened antagonistic strain Xh628A (GenBank No. PQ269269) and growth-promoting strain X5616A (GenBank No. PQ269268) were identified as Bacillus velezensis, and the induced antibacterial strain Xh649A (GenBank No. PQ269267) was identified as Acinetobacter pittii. The optimal addition ratio of the optimized flora was determined to be Xh628A:Xh649A:X5616A=5.0:1.0:4.5, and the average mycelial inhibition rate of the flora was 77.24 %, compared with the single strain, its growth-promoting and resistance-inducing ability has been improved. This study provides a research basis for the subsequent development of efficient, stable and environmentally friendly multifunctional biological pesticides, and provides a scientific method for the construction and ratio optimization of multifunctional biological flora.
WU Xu-Yan,LI Xing-Shan,CUI Chuan-Bin, et al. Screening, Construction and Optimization of Microbial Communities for Multifunctional Biocontrol of Tobacco Brown Spot[J]. 农业生物技术学报, 2025, 33(3): 628-641.
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