Abstract MYB transcription factor is one of the gene families with the largest number of members in plants, among which R2R3MYB plays an important role in anthocyanin synthesis. The study aimed to identify R2R3MYB gene family members at genomic level and analyze their functions in anthocyanin synthesis in Ginkgo biloba. The sequence information was obtained by homology alignment, and conserved domain analysis, evolutionary tree construction and conserved motif analysis were carried out by bioinformatics analysis software. The gene expression level was detected by qPCR. The gene function was studied through tobacco (Nicotiana benthamiana) leaf transformation. 78 R2R3MYB family members were identified in G. biloba genome, which were divided into 7 branches in the phylogenetic tree. One gene, Gb_34086, homologous to AtMYB75 of Arabidopsis thaliana, was selected and its expression was up-regulated by light induction. Gb_34086 protein was located in the nucleus and had transcriptional activation activity. The overexpression of Gb_34086 gene in tobacco leaves could enhance anthocyanin accumulation under light. This study preliminarily proved that Gb_34086 played a positive regulatory role in anthocyanin synthesis, which provides a basis for further study of the molecular mechanism of Gb_34086 and improving the the anthocyanin production of Ginkgo biloba.
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