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| Cloning and Activity Analysis of the CsCAT2 Promoter in Citrus sinensis |
| CHEN Ye, SHAN Si-Jie, ZHONG Lei-Jian, ZENG Wei, LIU Yu-Hang, LI Rui-Min* |
| College of Life Sciences, Gannan Normal University, Ganzhou 341000, China |
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Abstract Huanglongbing is a major impediment to the growth of the citrus industry. It is caused by Candidatus Liberibacter asiaticus which causes the production of reactive oxygen species in the phloem of citrus trees, resulting in cell death. The catalase (CAT) is essential for the elimination of hydrogen peroxide. In order to gain insights into the expression pattern of the catalase gene CsCAT2 in Citrus sinensis, the promoter of CsCAT2 was cloned, the cis-acting elements on the promoter were investigated, and truncations of the CsCAT2 promoter were constructed. Moreover, the response of the CsCAT2 promoter to various phytohormone treatments was evaluated. Analysis of the CsCAT2 promoter sequence revealed that it contained 15 cis-acting elements associated with biotic and abiotic stress and phytohormone signal response, predominantly for hormone signal response. The CsCAT2 promoter with its full-length had the highest promoter activity, and the activity of the truncated mutants decrease as their length decreasing. The activity of CsCAT2 promoter was significantly increased by abscisic acid, jasmonic acid, auxin, gibberellin and salicylic acid treatment, but treatment with ethephon significantly inhibited the activity of the CsCAT2 promoter. This study furnish data for further exploration of the regulatory process of the CsCAT2 gene from C. sinensis, and has broadened the theories concerning active oxygen scavenging metabolism during the interaction between Citrus spp. and Candidatus Liberibacter asiaticus.
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Received: 13 November 2023
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Corresponding Authors:
*liruimin@gnnu.edu.cn
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