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| Cloning, Expression and Protein Interaction Analysis of Drought-responsive Gene StGAMyb-like1 in Potato (Solanum tuberosum) |
| DENG Yu-Rong1,2, WEI Jing-Jing1,2, LIU Sheng-Yan1,3, YANG Liang1,3, WANG Xiao-Feng1,3, YANG Jiang-Wei1,2, ZHANG Ning1,2, SI Huai-Jun1,2,* |
1 State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China;
2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
3 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract R2R3-MYB transcription factors, as the largest subfamily of the MYB transcription factor family, play a crucial role in plant growth and development, material metabolism, and other processes. Additionally, they have the potential to enhance plant stress tolerance. In a previous study, a StGAMyb-like1 gene (GenBank No. XM_015303144), homologous to AtMYB33 in Arabidopsis thaliana, was identified in potato (Solanum tuberosum). The StGAMyb-like1 gene, a member of GAMYB subgroup 18 of R2R3-MYB, is capable of responding to drought stress. In this study, the StGAMyb-like1 gene was cloned from the potato variety 'Desiree', and its CDS was 1 572 bp. Bioinformatics analysis revealed that the promoter region of this gene contained multiple hormone response elements, and its protein sequence was similar to that of tomato (Solanum lycopersicum). The expression analysis in different tissues showed that the StGAMyb-like1 gene had higher expression level in leaves. The promoter sequence of the StCYP98A3 gene, which encodes coumarate 3-hydroxylase in the lignin synthesis pathway, was analyzed and found to have MYB binding site (MBS). It was confirmed that StGAMyb-like1 binded to the StCYP98A3 promoter and activated its transcription using yeast one hybrid, dual-luciferase reporter assay and β-glucuronidase (GUS) detection. This study provides a reference basis for further exploring the function of StGAMyb-like1 gene.
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Received: 27 December 2023
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Corresponding Authors:
*hjsi@gsau.edu.cn
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[1] 丁霄, 曹彩荣, 李朋波, 等. 2016. 植物木质素的合成与调控研究进展[J]. 山西农业科学, 44(9): 1406-1411.
(Ding X, Cao C R, Li P B, et al.2016. Research progress on synthesis and regulation of plant lignin[J]. Journal of Shanxi Agricultural Sciences, 44(9): 1406-1411.)
[2] 刘素军, 蒙美莲, 陈有君. 2018. 干旱胁迫及复水对马铃薯类黄酮合成途径中关键酶及基因表达的影响[J]. 植物生理学报, 54(1): 81-91.
(Liu S J, Meng M L, Chen Y J.2018. Effects of drought stress and rehydration on gene expression of key enzymes in the flavonoid biosynthesis pathway in potato[J]. Plant Physiology Journal, 54(1): 81-91.)
[3] 刘维刚, 唐勋, 付学, 等. 2022. 马铃薯抗旱性研究进展[J]. 中国马铃薯, 36(4): 358-369.
(Liu W G, Tang X, Fu X, et al.2022. Research progress in drought tolerance of potato[J]. Chinese Potato Journal, 36(4): 358-369.)
[4] Alber A V, Renault H, Basilio-Lopes A, et al.2019. Evolution of coumaroyl conjugate 3-hydroxylases in land plants: Lignin biosynthesis and defense[J]. The Plant Journal, 99(5): 924-936.
[5] Butt H I, Yang Z E, Gong Q, et al.2017. GaMYB85, an R2R3 MYB gene, in transgenic Arabidopsis plays an important role in drought tolerance[J]. BMC Plant Biology, 17(1): 142.
[6] Li J L, Han G L, Sun C F, et al.2019. Research advances of MYB transcription factors in plant stress resistance and breeding[J]. Plant Signaling & Behavior, 14(8): 1613131.
[7] Li J, Han G, Sun C, et al.2019. Research advances of MYB transcription factors in plant stress resistance and breeding[J]. Plant Signaling & Behavior, 14(8): 1613131.
[8] Li X R, Tang Y, Li H L, et al.2020. A wheat R2R3 MYB gene TaMpc1-D4 negatively regulates drought tolerance in transgenic Arabidopsis and wheat[J]. Plant Science, 299: 110613.
[9] Liu Y H, Shen Y, Liang M, et al.2022. Identification of peanut AhMYB44 transcription factors and their multiple roles in drought stress responses[J]. Plants, 11(24): 3522.
[10] Lv K W, Wei H R, Liu G F, et al.2021. A R2R3-MYB transcription factor gene, BpMYB123, regulates BpLEA14 to improve drought tolerance in Betula platyphylla[J]. Frontiers in Plant Science, 12: 791390.
[11] Patrizia G.2022. Genetics and evolution of abiotic stress tolerance in plants[J]. Genes, 13(8): 1380.
[12] Polle A, Chen S L, Eckert C, et al.2019. Engineering drought resistance in forest trees[J]. Frontiers in Plant Science, 9: 1875.
[13] Pratyusha D S, Sarada D V L.2022. MYB transcription factors-master regulators of phenylpropanoid biosynthesis and diverse developmental and stress responses[J]. Plant Cell Reports, 41(12): 2245-2260.
[14] Ramya M, Kwon O K, An H R, et al.2017. Floral scent: Regulation and role of MYB transcription factors[J]. Phytochemistry Letters, 19: 114-120.
[15] Schaart J G, Dubos C, Romero De La Fuente I, et al.2013. Identification and characterization of MYB-bHLH-WD40 regulatory complexes controlling proanthocyanidin biosynthesis in strawberry (Fragaria×ananassa) fruits[J]. The New Phytologist, 197(2): 454-467.
[16] Song Q, Kong L, Yang X, et al.2022. PtoMYB142, a poplar R2R3-MYB transcription factor, contributes to drought tolerance by regulating wax biosynthesis[J]. Tree Physiology, 42(10): 2133-2147.
[17] Song Z B, Zhao L, Ma W N, et al.2023. Ethylene inhibits ABA-induced stomatal closure via regulating NtMYB184-mediated flavonol biosynthesis in tobacco[J]. Journal of Experimental Botany, 74(21): 6735-6748.
[18] Stracke R, Werber M, Weisshaar B.2001. The R2R3-MYB gene family in Arabidopsis thaliana[J]. Current Opinion in Plant Biology, 4(5): 447-456.
[19] Wang N, Zhang W X, Qin M Y, et al.2017. Drought tolerance conferred in soybean (Glycine max L.) by GmMYB84, a Novel R2R3-MYB transcription factor[J]. Plant Cell Physiology, 58(10): 1764-1776.
[20] Wang, X P, Niu, Y L, Zheng, Y.2021. Multiple functions of MYB transcription factors in abiotic stress responses[J]. International Journal of Molecular Sciences, 22(11): 6125.
[21] Wu Y Q, Li T T, Cheng Z Y, et al.2021. R2R3-MYB transcription factor PlMYB108 confers drought tolerance in herbaceous peony (Paeonia lactiflora Pall.)[J]. International Journal of Molecular Sciences, 22(21): 11884.
[22] Wu Y, Wen J, Xia Y, et al.2022. Evolution and functional diversification of R2R3-MYB transcription factors in plants[J]. Horticulture Research, 9: uhac058.
[23] Yang J W, Zhang N, Mi X X, et al.2014. Identification of miR159s and their target genes and expression analysis under drought stress in potato[J]. Computational Biology and Chemistry, 53: 204-213.
[24] Yao C Y, Li X G, Li Y M, et al.2022. Overexpression of a Malus baccata MYB transcription factor gene MbMYB4 increases cold and drought tolerance in Arabidopsis thaliana[J]. International Journal of Molecular Sciences, 20(3): 1794.
[25] Yoon Y, Seo H D, Shin H, et al.2020. The role of stress-responsive transcription factors in modulating abiotic stress tolerance in plants[J]. Agronomy, 10(6): 788.
[26] Yu Y H, Bi C X, Wang Q, et al.2019. Overexpression of TaSIM provides increased drought stress tolerance in transgenic Arabidopsis[J]. Biochemical and Biophysical Research Communications, 512(1): 66-71.
[27] Zhang C Y, Liu H C, Zhang X S, et al.2020. VcMYB4a, an R2R3-MYB transcription factor from Vaccinium corymbosum, negatively regulates salt, drought, and temperature stress[J]. Gene, 757: 144935. |
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