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| Genetic Diversity Analysis of Cauliflower (Brassica oleracea var. botrytis) Breeding Germplasm Based on SSR Markers |
| SONG Xiao-Yu1,2, HAN Shuo1, GAN De-Fang2, SONG Shun-Hua1, LIU Fan1, GUO Ning1,*, MENG Shu-Chun1,* |
1 State Key Laboratory of Vegetable Biobreeding/National Engineering Research Center for Vegetables/ Inspection and Test Center of Vegetable Seed Quality of Ministry of Agriculture and Rural Affairs/Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China;
2 Horticulture College, Anhui Agricultural University, Anhui 230036, China |
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Abstract Cauliflower (Brassica oleracea var. botrytis), originating from Europe, has a relatively narrow genetic diversity in China's germplasm resources, urgently requiring studies on germplasm innovation and evaluation techniques. The creation of double haploids (DH) using microspore culture not only shortens the breeding cycle but is also a crucial means for germplasm innovation. Understanding and evaluating the genetic diversity of cauliflower microspore progeny is a key issue to be resolved for the application of DH technology in germplasm innovation and breeding. In this study, 29 pairs of SSR primers with clear and stable band patterns and good polymorphism were selected, with PIC range of 0.29 to 0.57 and average value of 0.42. Using these 29 primers, the marker genotyping study was conducted on a cauliflower breeding germplasm population consisting of 66 DH lines and 18 advanced inbred lines, totaling 84 materials. The results showed that the genetic distances of the materials mainly ranged from 0.3 to 0.6, with an average of 0.56; the genetic similarity coefficients ranged from 0.50 to 0.97, with an average of 0.57. At a genetic similarity coefficient of 0.55, the materials were divided into 4 groups. Through the analysis of the relationships of DH materials, it was found that high genetic diversity could be obtained in the DH progenies of F1 hybrid, confirming that DH breeding could serve as an important tool for germplasm innovation in cauliflower. This study provides theoretical and technical support for the evaluation and utilization of genetic diversity in cauliflower.
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Received: 27 July 2023
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Corresponding Authors:
* mengshuchun@nercv.org; guoning@nercv.org
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[1] 林珲, 朱海生, 陈敏氡, 等. 2014. 花椰菜游离小孢子培养技术研究进展[J]. 中国农学通报, 30(13): 71-75.
(Lin H, Zhu H S, Chen M D, et al.2014. Breeding present situation and the isolated microspore culture research progress in cauliflower[J]. Chinese Agricultural Science Bulletin, 30(13):71-75.)
[2] 陈琛, 庄木, 李康宁, 等. 2010. 甘蓝EST-SSR标记的开发与应用[J]. 园艺学报, 37(2): 221-228.
(Chen S, Zhuang M, Li K N, et al.2010. Development and utility of EST-SSR marker in cabbage[J]. Acta Horticulturae Sinica, 37(2): 221-228.)
[3] 陈琛, 张兴桃, 程斐, 等. 2011. 秋甘蓝品种的SSR指纹图谱的构建[J]. 园艺学报, 38(01): 159-164.
(Chen S, Zhang X T, Cheng P, et al.2011. Establishment of SSR fingerprinting on autumn cabbage hybrids and their parents[J]. Acta Horticulturae Sinica, 38(01): 159-164.)
[4] 褚云霞, 邓姗, 李寿国, 等. 2020. 花菜类SSR分子标记筛选及其在品种鉴定上的应用[J]. 分子植物育种, 20(01): 163-175.
(Chu Y X, Deng S, Li S G, et al.2020. Validation of microsatellite markers and its application for varieties identification in cauliflower and broccoli[J]. Molecular Plant Breeding, 20(01): 163-175.
[5] 丁燕, 木万福, 杨龙, 等. 2021. 花菜类杂交种纯度鉴定SSR核心引物筛选[J]. 中国种业, (11): 79-84.
(Ding Y, Mu W F, Yang L, et al. 2021. Screening of SSR core primers for purity identification of cauliflower hybrids[J]. China Seed Industry, (11): 79-84.)
[6] 樊文强, 盖红梅, 孙鑫, 等. 2016. SSR数据格式转换软件DataFormater[J]. 分子植物育种, 14(01): 265-270.
(Fan W Q, Gai H M, Sun X, et al.2016. Data Formater, a software for SSR data formatting to develop population genetics analysis[J]. Molecular Plant Breeding, 14(01): 265-270.)
[7] 郭宁, 高怀杰, 韩硕, 等. 2017. 观赏羽衣甘蓝SSR标记分型与亲缘关系研究[J]. 植物遗传资源学报, 18(02): 349-357+366.
(Guo N, Gao H J, Han S, et al.2017. Genotypic and genetic relationship analysis of ornamental kale (Brassica oleracea var. acephala) by SSR marker[J]. Journal of Plant Genetic Resources, 18(02): 349-357, 366.)
[8] 韩硕, 郭宁, 张月云, 等. 2018. 羽衣甘蓝双单倍体育种技术研究[J]. 农业生物技术学报, 26(03): 521-529.
(Han S, Guo N, Zhang Y Y, et al.2018. Researches on the double haploid breeding of ornamental kale (Brassica oleracea var. acephala)[J]. Journal of Agricultural Biotechnology, 26(03): 521-529.)
[9] 刘春晴, 李光庆, 姚雪琴, 等. 2018. 基于SSR标记的花菜类种质亲缘关系和群体遗传结构分析[J]. 分子植物育种, 16(4): 1327-1337.
(Liu C Q, Li G Q, Yao X Q, et al.2018. Analysis of genetic diversity and population structure in Brassica oleracr based on SSR markers[J]. Molecular Plant Breeding, 16(4): 1327-1337.)
[10] 苗明军. 2010. SSR标记在甘蓝遗传多样性及杂种鉴定中的应用研究[D]. 硕士学位论文, 西南大学, 导师: 李成琼, pp. 17-26.
(Miao M J.2010. Application of SSR marker on cabbage germplasm genetic diversity and purity identification[D]. Thesis for M. S., Southwest University, Supervisor: Li C Q, pp. 17-26.)
[11] 孟艳. 2021. 大白菜种质资源多样性及炮弹形大白菜杂种优势研究及杂种纯度鉴定[D]. 硕士学位论文, 西北农林科技大学, 导师: 张鲁刚, pp. 10-13.
(Meng Y.Study on diversity of chinese cabbage germplasm resource and heterosis of cannonball-shaped chinese cabbage and purity identification of an excellent hybrids[D]. Thesis for M. S., Northwest A&F University, Superivsor: Zhang L G, pp. 10-13.)
[12] 盛小光, 赵振卿, 王建升, 等. 2019. 基于SSR标记的花椰菜和青花菜遗传多样性分析[J]. 植物遗传资源学报, 20(04): 949-959.
(Sheng X G, Zhao Z Q, Wang J S, et al.2019. Genetic diversity analysis of cauliflower and broccoli based on SSR markers[J]. Journal of Plant Genetic Resources, 20(04): 949-959.)
[13] 孙继峰, 刘玉梅, 方智远, 等. 2012. 青花菜相同亲本的DH与F2群体遗传多样性的比较[J]. 园艺学报, 39(06): 1090-1098.
(Sun J F, Liu Y M, Fang Z Y, et al.2012. The comparison analysis of genetic diversity between DH population and F2 population derived form the same two parents in Brassica oleracea var. italica[J]. Acta Horticulturae Sinica, 39(06): 1090-1098.)
[14] 王冬梅. 2011. 甘蓝类作物亲缘关系的SSR分析[D]. 硕士学位论文, 中国农业科学院, 导师: 庄木, pp. 22-32.
(Wang D M.2011. Analysis of genetic relationships of Brassica oleracea based on SSR marker[D]. Thesis for M. S., Chinese Academy of Agricultural Sciences, Supervisor: Zhuang M, pp. 22-32.)
[15] 王庆彪, 张扬勇, 庄木, 等. 2014. 中国50个甘蓝代表品种EST-SSR指纹图谱的构建[J]. 中国农业科学, 47(01): 111-121.
(Wang Q B, Zhang Y Y, Zhuang M, et al.2014. EST-SSR fingerprinting of fifty cabbage representative varieties from china[J]. Scientia Agricultura Sinica, 47(01): 111-121.)
[16] 王永飞, 王鸣, 郑学勤, 等. 2000. 提取大白菜基因组DNA的几种方法比较[J]. 西北农业大学学报, 04: 85-88.
[17] 王志奎. 2019. 48 对水稻分子指纹SSR标记与杂种优势相关性研究[D]. 硕士学位论文, 湖南师范大学, 导师: 梁满中, pp. 22-26.
(Wang Z K.2019.The correlation between 48 pairs of molecular fingerprinting SSR markers and heterosis in rice[D]. Thesis for M. S., Hunan Normal University, Supervisor: Liang M Z, pp. 22-26.)
[18] 朱焕焕. 2019. 我国花椰菜种业发展现状、市场需求及研究热点综述[J]. 蔬菜, (04): 30-35.
(Zhu H H. 2019. Summary of the current situation,market demand and research hotspots of cauliflower seed industry in China[J]. Vegetables, (04): 30-35.)
[19] 朱世杨. 2019. 不同来源CMS 应用于花椰菜杂种优势的研究[D]. 博士学位论文, 福建农林大学, 导师: 周元昌, pp. 51-61.
(Zhu S Y.2019. Studies on the different CMS applied in the heterosis of cauliflower (Brassica oleracea var. botrytis)[D]. Thesis for Ph. D., Fujian Agriculture and Forestry University, Supervisor: Zhou Y C, pp. 51-61.)
[20] Adeyemo O, Menkir A, Melaku G, et al.2011. Genetic diversity assessment and relationship among tropicalyellow endosperm maize inbred lines using SSR markers[J]. Maydica, 56(1): 43-49.
[21] Guo N, Wang S, Wang T, et al.2024. A graph-based pan-genome of Brassica oleracea provides new insights into its domestication and morphotype diversification[J]. Plant Communications, 12;5(2).
[22] Liu K, Muse S V.2005. PowerMarker: an integrated analysis environment for genetic marker analysis[J]. Bioinformatics, 21(9): 2128-2129.
[23] Krawczak M, Nikolaus S, Von Eberstein H, et al.2006. PopGen: Population-based recruitment of patients and controls for the analysis of complex genotype-phenotype relationships[J]. Community Genetics, 9(1): 55-61.
[24] Nadeem M A, Nawaz M A, Shahid M Q, et al.2018. DNA molecular markers in plant breeding: Current status and recent advancements in genomic selection and genome editing[J]. Biotechnology & Biotechnological Equipment, 32(2): 261-85.
[25] Pattanaik A, Reddy D C L, Ramesh S, et al.2018. Comparison of traditional GOT and DNA-based PCR assay to estimate F1 hybrid purity in cauliflower[J]. Current Science, 115(11): 2012-2095.
[26] Ram H, Sureja A K, Kriahnan S G, et al.2016. Diversity analysis in snowball cauliflower (Brassica oleracea var. botrytis) using microsatellite markers[J]. Indonesian Journal of Biotechnology, 86(10): 1299-1304.
[27] Verma V K, Kalia P, Prasanna B M.2017. Genetic characterization of self-incompatible lines and strategies for heterosis breeding in cauliflower[J]. International Journal of Vegetable Science, 23(5): 411-429.
[28] Zhao Z Q, Gu H H, Sheng X G, et al.2014. Genetic diversity and relationships among loose-curd cauliflower and related varieties as revealed by microsatellite markers[J]. Scientia Horticulturae, 166(1): 105-110.
[29] Zhu S Y, Zhang X L, Liu Q, et al.2018. The genetic diversity and relationships of cauliflower (Brassica oleracea var. botrytis) inbred lines assessed by using SSR markers[J]. PLOS ONE, 13(2): e0208551. |
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