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| Identification and Expression Analysis of FRK Gene Family in Cucumber (Cucumis sativus) |
| MA Juan-Juan1, HOU Dong1,2,*, HUANG Shu-Chao1, ZHANG Dong-Qin2, YUE Hong-Zhong2, LI Ya-Li2, XIE Jian-Ming1 |
1 College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China;
2 Vegetable Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China |
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Abstract Fructokinase (FRK) is the main catalytic enzyme in the phosphorylation of fructose and plays a key role in the regulation of plant growth and development. In order to further study the FRK gene function of Cucumis sativus, the FRK gene family and its molecular characteristics were systematically identified and analyzed, and the gene expression patterns under abiotic stress were analyzed. The results showed that there were 12 FRK genes distributed on 7 chromosomes. The number of encoded amino acids was 331~588, and the molecular weight was 35.63~65.90 kD. The FRK gene family of cucumber was divided into 3 subfamilies by phylogenetic analysis, and the gene structure and motif in each subfamily were basically the same. The promoter sequences mainly contained cis-acting elements such as light response, low temperature response, drought response, hormone response, defense stress and plant growth and development. Tissue-specific expression analysis showed that the expression levels of some FRK genes in cucumber were low or not expressed in roots, stems, leaves, female flowers, and male flowers, indicated that different cucumber FRK genes had tissue-specific expression patterns. Abiotic stress expression analysis showed that CsaFRK6, CsaFRK8 and CsaFRK12 was significantly up-regulated under low temperature stress (P<0.05). The expression of CsaFRK2, CsaFRK5 and CsaFRK7 was significantly up-regulated under salt stress (P<0.05). CsaFRK1~CsaFRK9 were significantly up-regulated under drought stress (P<0.05). The expression patterns of FRK genes in different tissues and organs were different in response to abiotic stress. This study provides a reference for elucidating the biological gene function of cucumber and its response to abiotic stress.
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Received: 20 August 2023
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Corresponding Authors:
* houdong215@163.com
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[1] 高丽红, 眭晓蕾, 齐艳花, 等. 2018. 日光温室黄瓜越冬长季节栽培高产关键理论与技术[J]. 中国蔬菜, (10): 1-6.
(Gao L H, Gui X L, Qi Y H, et al. 2018. Key theories and techniques of cucumber overwintering long season cultivation for high yield in solar greenhouse[J]. Chinese Vegetables, (10): 1-6.)
[2] 蒋静静, 屈锋, 苏春杰, 等. 2019. 不同肥水耦合对黄瓜产量品质及肥料偏生产力的影响[J]. 中国农业科学, 52(1): 86-97.
(Jiang J J, Qu F, Su C J, et al.2019. Effects of different fertilizer and water coupling on cucumber yield and quality and fertilizer partial productivity[J]. Agricultural Sciences in China, 52(1): 86-97.)
[3] 林德佩. 2017. 黄瓜植物的起源和分类研究进展[J]. 中国瓜菜, 30(7): 1-3.
(Lin D P.2017. Advances in the origin and classification of cucumber plants[J]. Chinese Melon Vegetable, 30(7): 1-3.)
[4] 秦巧平, 张上隆, 陈俊伟, 等. 2004. 温州蜜柑果实发育期间果糖激酶与糖积累的关系[J]. 植物生理与分子生物学学报, 30(4): 435-440.
(Qin Q P, Zhang S L,Chen J W,et al.The relationship of fructokinase and sugar accumulation during fruit development in satsuma mandarin[J]. Physiology and Molecular Biology of Plants, 30(4): 435-440.)
[5] 王卫平, 崔娜, 于志海, 等. 2011. 番茄果实果糖激酶基因的克隆及其表达分析[J]. 华北农学报, 26(3): 11-15.
(Wang W P, Cui N, Yu Z H, et al.2011. Cloning and expression analysis of fructokinase gene (FRK) from tomato fruit[J]. Acta Agriculturae Boreali-Sinica, 26(3): 11-15.)
[6] 徐雅菲, 孙宇蛟, 崔浩楠, 等. 2019. 甜瓜属 FRK 基因家族的鉴定与特征分析[J].分子植物育种, 17(15): 4954-4960.
(Xu Y F, Sun Y J, Cui H N, et al.2019. Identification and characteristic analysis of FRK gene family in Cucumi[J]. Molecular Plant Breeding, 17(15): 4954-4960.)
[7] 叶香媛, 周文彬. 2021. 植物果糖激酶研究进展[J]. 科学通报, 66(22): 2820 -2831.
(Ye X Y, Zhou W B. Update on fructokinase in higher plants[J]. Chinese Science Bulletin, 66(22): 2820-2831.)
[8] 余炳伟, 董日月, 雷建军, 等. 2017. 黄瓜耐热材料的筛选与鉴定[J]. 分子植物育种, 15(11): 4695-4705.
(Yu B W,Dong R Y, Lei J J, et al.2017. Screening and identification of heat resistant materials in cucumber[J]. Molecular Plant Breeding, 15(11): 4695-4705.)
[9] 张然然, 郝丽宁, 单素兰, 等. 2015. 不同结果部位黄瓜果实芳香物质含量和成分及相关因素分析[J]. 农业生物技术学报, 23(8): 1031~1039.
(Zhang R R, Hao L N, Shan S L, et al.2015. Analysis on the content and components of aromatic substances and related factors in cucumber fruits from different fruiting-parts[J]. Journal of Agricultural Biotechnology, 23(8): 1031-1039.)
[10] 张圣平, 顾兴芳. 2020. 黄瓜重要农艺性状的分子生物学[J].中国农业科学, 53(1): 117-121.
(Zhang S P, Gu X F.2020. Molecular biology of important agronomic traits in cucumber[J]. Agricultural Sciences in China, 53(1): 117-121.)
[11] 张钊. 2016. 山梨醇和蔗糖在苹果抵抗干旱胁迫中的作用研究[D]. 硕士学位论文, 西北农林科技大学, 导师: 李明军, pp. 37-44.
(Zhang Z.Effects of sorbitol and sucrose on resistance to drought stress in apples[D]. Thesis for M.S., Northwest Agriculture & Forestry University, Supervisor: Li M J, pp. 37-44.)
[12] 赵建华, 尹跃, 李浩霞, 等. 2018. 枸杞果糖激酶基因LbFRK7的克隆及表达分析[J]. 西北植物学报, 38(5): 816-822.
(Zhao J H, Yin Y, Li H X, et al.2018. Cloning and expression analysis of fructokinase gene (LbFRK7) from wolfberry (Lycium barbarum Linn.)[J]. Acta Botanica Boreali-Occidentalia Sinica, 38(5): 816-822.)
[13] Fulda S, Mikkat S, Stegmann H, et al.2011. Physiology and proteomics of drought stress acclimation in sunflower (Helianthus annuus L.)[J]. Plant Biology, 13(4): 632-642.
[14] Granot D, Kelly G, Stein O, et al.2014. Substantial roles of hexokinase and fructokinase in the effects of sugars on plant physiology and development[J]. Journal of Experimental Biology, 65(3): 809-19.
[15] Jiang H, Dian W, Liu F, et al.1992. Isolation and characterization of two fructokinase cDNA clones from rice[J]. Phytochemistry, 1(1): 5-1027.
[16] Kanayama Y, Dai N, Granot D, et al.1997. Divergent fructokinase genes are differentially expressed in tomato[J]. Plant Physiology, 113(4): 1379-84.
[17] Klotz K L, Finger F L, Anderson M D.2006. Wounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet root[J]. Postharvest Biology and Technology, 41(1): 48-55.
[18] Qin Q, Zhang S, Chen J, et al.2004. Isolation and expression analysis of fructokinase genes from Citrus[J]. Acta Botanica Sinica, 46(12): 1408-1414.
[19] Qin Q P, Cui Y Y, Zhang L L, et al.2014. Isolation and induced expression of a fructokinase gene from loquat[J]. Russian Journal of Plant Physiology, 61(3): 289-297.
[20] Smith S B, Taylor M A, Burch L R, et al.1993. Primary structure and characterization of a cDNA clone of fructokinase from potato (Solanum tuberosum L. cv. record)[J]. PlantPhysiology, 102(3): 1043-1043.
[21] Zhang S, Nichols S E, Dong J G.2003. Cloning and characterization of two fructokinases from maize[J]. Plant Science, 165(5): 1051-1058.
[22] Zorb C, Schmitt S, Muhling K H.2010. Proteomic changes in maize roots after short-term adjustment to saline growth conditio[J]. Proteomics, 10(24): 4441-4449. |
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