Cloning and Expression Analysis of the ghr Gene in Channa maculata and Its Response to Sexual Steroid Hormone Induction
WU Yu-Xia1,2, ZHANG Xiao-Tian1,2, ZHANG Yang1,3, ZHAO Jian1,2, LUO Qing1, LIU Hai-Yang1, FEI Shu-Zhan1, CHEN Kun-Ci1,2, OU Mi1,3,*
1 Pearl River Fisheries Research Institute/Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Guangzhou 510380, China; 2 College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China; 3 School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan 411201, China
Abstract The growth hormone (GH)-insulin-like growth factor (IGF) axis is the main endocrine axis that regulates fish growth. In order to explore the regulatory mechanism of growth hormone receptor (GHR) in the growth difference between female and male blotched snakehead (Channa maculata), the ghr gene in C. maculata was cloned, gene structure was analyzed, and the expression patterns of ghr in different adult tissues and liver at different developmental stages were clarified, meanwhile, its response to exogenous sex steroid hormones were also analyzed. The results showed that 2 602 bp cDNA sequence of the ghr gene in C. maculata was obtained, including 100 bp 5'-UTR, 1 872 bp ORF, encoding 623 amino acids, and 630 bp 3'-UTR. The genome sequence of ghr gene in C. maculata was 18 118 bp long, including 9 exons and 8 introns. 899 bp 5'-flanking sequence was identified, which contained androgen receptor (AR), bromomain-containing protein 4 (BRD4) and suppressor element-1-silencing transcription factor (REST) and other transcription factor binding sites. ghr was highly expressed in the liver of adult C. maculata, and its expression in males was significantly higher than that in females (P<0.01). The expression analysis of ghr gene at different developmental stages displayed that the highest expression of ghr gene was found in the liver of 365 d male C. maculata, which was significantly higher than that in females at the same period (P<0.01). Hormone induction experiments showed that long-term injection of 17α-ethynylestradiol (EE2) inhibited ghr expression in male and female C. maculata, long-term injection of 17α-methyltestosterone (MT) inhibited ghr expression in female C. maculata, but promoted ghr expression in males within a certain period of time. The results demonstrate that GHR might play a key role in the growth difference between females and males and it is also regulated by sex steroid hormones in C. maculata. This study provides the reference for further exploring the molecular mechanism of growth regulation in blotched snakehead.
WU Yu-Xia,ZHANG Xiao-Tian,ZHANG Yang, et al. Cloning and Expression Analysis of the ghr Gene in Channa maculata and Its Response to Sexual Steroid Hormone Induction[J]. 农业生物技术学报, 2024, 32(6): 1371-1384.
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