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| Identification of MYB Gene Family in Strawberry (Fragaria vesca) and Expression Analysis at Different Coloration Stages of Fruits |
| LIANG Wei1, WANG Hai1, FENG Yong-Qing1, ZHANG Ao-Ning1, TIAN Xue-Chun1, NAN Xin-Tong1, LI Wen-Fang1, LIU Fei-Yun2, MA Zong-Huan1,* |
1 Horticulture College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China;
2 Lanzhou Jinyisheng Agricultural Science and Technology Company, Lanzhou 730060, China |
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Abstract Fruit coloration is mainly determined by flavonoids such as anthocyanins, and MYB transcription factors play an important role in the regulation of anthocyanins. In order to explore the effect of strawberry (Fragaria vesca) MYB gene family on strawberry fruit coloring, in this study, 114 MYB gene family members were identified by homology alignment of the MYB gene family sequences in the Arabidopsis thaliana and strawberry gene databases. The chromosomal positioning, physicochemical properties, subcellular localization and secondary structure analysis of the strawberry MYB gene family were analyzed by bioinformatics analysis software to determine the naming of the genes and the location of the main expression. The gene family was classified and its functions were predicted based on collinearity, gene structure, and promoter cis-acting element prediction. The results showed that MYB family members were predominantly expressed in the nucleus, and all but FvMYB7 were hydrophilic proteins. Among the 114 members, 107 proteins exhibited interactions with each other. A relative synonymous codon usage value was greater than 2.0. This indicated that there were highly preferred codons in strawberry MYB gene. 114 members could be divided into 3 subgroups according to the phylogenetic tree. Except FvMYB72 which didn't contain any action elements, all other members contained light, abiotic stress, hormone and meristem response elements. qRT-PCR analysis indicated that most members were in the S3 (50% coloring period) with high relative expression levels, however, the expressions of FvMYB33, FvMYB57, and FvMYB75 were higher in the S4 (full coloring stage). The tissue-specific expression profile results showed that only the FvMYB33 was specifically expressed in the fruit peel, and the results of anthocyanin content showed the highest content was in S4 period. It was speculated that FvMYB33 was the main gene regulating strawberry fruit coloring. This study provides a reference to further explore the mechanism of MYB gene family in strawberry fruit coloring.
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Received: 17 July 2023
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Corresponding Authors:
* mazohu@163.com
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[1] 常博, 刘聪, 刘莉, 等. 2019. MdMYB1启动子甲基化对不同色泽类型苹果品种果皮花青苷合成的调控作用[J]. 分子植物育种印刷版, 16(22): 7415-7422.
(Chang B, Liu C, Liu L, et al.2019. Regulatory effects of MdMYB1 promoter methylation on anthocyanin synthesis in apple peel of different color types[J]. Molecular Plant Breeding Print Edition, 16(22): 7415-7422.)
[2] 丁体玉. 2021. MicroRNA172和MdMYB28调控苹果花青苷合成的分子机制研究[D]. 博士学位论文, 华中农业大学, 导师: 刘崇怀, 刘继红. pp. 6-10.
(Ding T Y.2021. Molecular mechanisms of MicroRNA172 and MdMYB28 regulating the synthesis of apple anthocyanin[D]. Thesis for PhD., Huazhong Agricultural University, Tutors: Liu C H, L J H. pp. 6-10.)
[3] 郭丽丽, 卢世雄, 乃国洁, 等. 2022. 草莓PYL基因家族的鉴定与非生物胁迫下的表达分析[J]. 农业生物技术学报, 30(12): 2315-2332.
(Guo L L, Lu S X, Nai G J, et al.2022. Identification and expression analysis of PYL gene family in strawberry under abiotic stress[J]. Chinese Journal of Agricultural Biotechnology, 30(12): 2315-2332.)
[4] 关雎. 2018. 汉源黑樱桃MYB10基因的克隆、表达及功能验证[D]. 硕士学位论文, 四川农业大学, 导师: 吕秀兰. pp. 45.
(Guan J.2018. Cloning, expression and functional verification of MYB10 gene in Hanyuan black cherry[D]. Thesis for M.S., Sichuan Agricultural University, Supervisor: Lv X L. pp. 45.)
[5] 贺涵, 刘传和, 邵雪花, 等. 2022. 菠萝R2R3-MYB基因家族S20亚族的鉴定[J]. 西北农林科技大学学报(自然科学版), 50(12): 117-127.
(He H, Liu C H, Shao X X, et al.2022. Identification of the S20 subfamily of the R2R3-MYB gene family in pineapple[J]. Journal of Northwest A&F University (Natural Science Edition), 50(12): 117-127.)
[6] 焦棒棒. 2021. AcMYB306调控洋葱花青苷合成的分子机理研究[D]. 硕士学位论文,河南农业大学, 导师: 马长生. pp. 31-32.
(Jiao B B.2021. Molecular mechanism of AcMYB306 regulation of onion anthocyanin synthesis[D]. Thesis for M.S., Henan Agricultural University, Advisor: Ma C S.pp. 31-32.)
[7] 李泊霖. 2022. 不同果色树莓MYB10基因促进花色苷形成功能研究[D]. 硕士学位论文, 东北农业大学, 导师: 杨国慧. pp. 29.
(Li B L.2022. Function of MYB10 gene promoting anthocyanin formation in raspberries with different fruit colors[D]. Thesis for M.S., Northeast Agricultural University, Supervisor: Yang G H, pp. 29.)
[8] 李军, 赵爱春, 刘长英, 等. 2016. 桑树花青素合成相关MYB类转录因子的鉴定与功能分析[J].西北植物学报, 36(06): 1110-1116.
(Li J, Zhao A C, Liu C Y, et al.2016.Identification and functional analysis of MYB-type transcription factors related to anthocyanin synthesis in Mulberry[J]. Acta Botanica Sinica, 36(06): 1110-1116.)
[9] 李鲁华, 王忠妮, 任明见, 等. 2022. 谷类作物中植物激素调控花青素合成的研究进展[J]. 山地农业生物学报, 41(04): 62-66.
(Li L H, Wang Z N, Ren M J, et al.2022. Research progress of plant hormones regulating anthocyanin synthesis in cereal crops[J]. Journal of Mountain Agricultural Biology, 41(04): 62-66.)
[10] 李明霞, 张楚楚, 云欠, 等. 2005. 茭白MYB基因家族鉴定及表达模式分析[J]. 分子植物育种, 10(5): 236-242.
(Li M X, Zhang C C, Yun Q, et al.2005. Family identification and expression pattern analysis of MYB gene in wild rice stem[J]. Molecular Plant Breeding, 10(5): 236-242.)
[11] 李慎昌. 2020. VdMYB14调控刺葡萄果实花色苷的合成[D]. 硕士学位论文, 中国农业科学院, 导师: 刘崇怀. pp. 1-60.
(Li S C.2020. Regulation of anthocyanin synthesis by VdMYB14 in grapevine[D]. Thesis for M.S., Chinese Academy of Agricultural Sciences, Supervisor: Liu C H. pp. 1-60.)
[12] 李潇, 郭文芳, 杨莉, 等. 2023. 柑橘抗逆基因MYB96的克隆与表达分析[J]. 华北农学报, 38(02): 57-64.
(Li X, Guo W F, Yang L, et al.2023. Cloning and expression analysis of stress resistance gene MYB96 in citrus[J]. Journal of North China Agricultural Sciences, 38(02): 57-64.)
[13] 吕运舟, 董筱昀, 梁珍海, 等. 2021. 转录组的黄山栾树MYB基因家族鉴定及表达[J]. 东北林业大学学报, 49(04): 33-38.
(Lv Y Z, Dong X Y, LIANG Z H, et al.2021. Transcriptomic identification and expression of MYB gene family in Koelreuteria huangshan[J]. Journal of Northeast Forestry University, 49(04): 33-38.)
[14] 彭亚丽, 高倩, 董文, 等. 2020. MYB转录因子调控蔬菜花青素生物合成的研究进展[J]. 中国瓜菜, 33(12): 1-7.
(Peng Y L, Gao Q, Dong W, et al.2020. Research progress of MYB transcription factor regulating anthocyanin biosynthesis in vegetables[J]. Chinese Gourami,2020, 33(12): 1-7.)
[15] 齐宇. 2020. MY-bHLH-WD40对牡丹花青苷生物合成的转录调控机制[D]. 博士学位论文, 中国林业科学研究院, 导师: 王雁. pp. 128-134.
(Qi Y.2020. Transcriptional regulation mechanism of MYB--bHLH--WD40 on moranin biosynthesis[D]. Thesis for PhD., Chinese Academy of Forestry, Supervisor: Wang Y. pp. 128-134.)
[16] 阙秋霞, 赖恭梯, 潘若, 等. 2023. 刺葡萄MYB基因家族鉴定及其在不同光质下的表达模式分析[J]. 农业生物技术学报, 30(02): 298-310.
(Que Q X, LAI G T, Pan R, et al.2023. Identification of MYB gene family and analysis of its expression pattern under different light quality in Vitis acanthophora[J]. Chinese Journal of Agricultural Biotechnology, 30(02): 298-310.)
[17] 苏梦月. 2023. 莲调控花青素生物合成的MYB转录因子鉴定与功能解析[D]. 硕士学位论文, 湖北大学, 导师: 杨平仿. pp. 41-42.
(Su M Y.2023. Identification and functional analysis of MYB transcription factors regulating anthocyanin biosynthesis in lotus[D]. Thesis for M.S., Hubei University, Supervisor: Yang P F, pp. 41-42.)
[18] 王世界. 2016. 茄子花青素生物合成关键MYB转录因子的筛选及克隆[D]. 硕士学位论文, 山东农业大学, 导师: 杨凤娟. pp. 42-46.
(Wang S J.2016. Screening and cloning of MYB transcription factors key to anthocyanin biosynthesis in eggplant[D]. Thesis for M.S., Shandong Agricultural University, Supervisor: Yang F J, pp. 42-46.)
[19] 王文涛. 2021. 黄芩种质资源收集评价及R2R3-MYB家族成员的初步研究[D]. 硕士学位论文, 陕西师范大学, 导师: 曹晓燕. pp. 63-64.
(Wang W T.2021. Collection and evaluation of germplasm resources of Scutellaria baicalensis and preliminary study on members of the R2R3-MYB family[D]. Thesis for M.S., Shaanxi Normal University, Supervisor: Cao X Y, pp. 63-64.)
[20] 王晓珊, 葛晨辉, 康亚妮, 等. 2020. 菠菜转录因子MYB基因家族的鉴定及表达分析[J]. 上海师范大学学报(自然科学版), (06): 677-691.
(Wang X S, Ge C H, Kang Y Y, et al.2020. Identification and expression analysis of transcription factor MYB gene family in spinach[J]. Journal of Shanghai Normal University (Natural Science), (06): 677-691.)
[21] 吴瑞, 林定, 罗栋, 等. 2022. 番红花R2R3-MYB转录因子的鉴定与时空表达分析[J]. 农业生物技术学报, 30(03): 457-472.
(Wu R, Lin D, Luo D, et al.2022. Identification and spatiotemporal expression of R2R3-MYB transcription factor in Saffron[J]. Chinese Journal of Agricultural Biotechnology, 30(03): 457-472.)
[22] 肖竞淇, 陈怡, 谢岩, 等. 2021. 桑树miR858及MYB转录因子基因在桑椹花青素合成过程中的表达分析[J]. 蚕业科学, 47(06): 501-509.
(Xiao J Q, Chen Y, Xie Y, et al.2021. Expression analysis of miR858 and MYB transcription factors in mulberry anthocyanin synthesis[J]. Science of Sericulture, 47(06): 501-509.)
[23] 许海峰, 王中堂, 陈新, 等. 2022. 冬枣果皮着色相关类黄酮靶向代谢组学及潜在MYB转录因子分析[J]. 园艺学报, 49(08): 1761-1771.
(Xu H F, Wang Z T, Chen X, et al.2022. Metabolomics of flavonoid targeting and potential MYB transcription factors in winter jujube peel coloring[J]. Chinese Journal of Horticulture, 49(08): 1761-1771.)
[24] 徐荣荣, 常青, 魏莹, 等. 2023. 水稻MYB基因家族特征及其在RGSV和RRSV侵染后的响应[J]. 物病理学报, 53(02): 258-266.
(Xu R R, Chang Q, Wei Y, et al.2023. Characteristics of rice MYB gene family and its response to RGSV and RRSV infection[J]. Chinese Journal of Biological Pathology, 53(02): 258-266.)
[25] 张耐. 2022. 甘草中调控甘草酸生物合成的MYB基因家族分析与挖掘[D]. 硕士学位论文, 兰州交通大学, 导师: 阎侃, pp. 1-63.
(Zhang N.2022. Analysis and mining of MYB gene family regulating glycyrrhizic acid biosynthesis in licorice[D]. Thesis for M.S., Lanzhou Jiaotong University, Supervisor: Yan K, pp.1-63.)
[26] 张云婷. 2019. 光质和低温对草莓果实花青素合成的影响及其分子机制[D]. 博士学位论文, 四川农业大学, 导师: 汤浩茹. pp. 1-3.
(Zhang Y T.2019. Effects of light quality and low temperature on anthocyanin synthesis in strawberry fruit and its molecular mechanism[D]. Thesis for PhD., Sichuan Agricultural University, Supervisor: Tang H R, pp. 1-3.)
[27] 左鑫. 2022. MYB转录因子调控3种地黄属植物花青素合成的分子机制研究[D]. 硕士学位论文, 河南农业大学, 导师: 王丰青. pp. 57.
(Zuo X.2022. Molecular mechanism of anthocyanin synthesis in three Rehmannia plants regulated by MYB transcription factor[D]. Thesis for M.S., Henan Agricultural University, Supervisor: Wang F G, pp. 57.)
[28] Feng Y Q, Yang S W, Li W F, et al.2023. Genome-wide identification and expression analysis of ANS family in strawberry fruits at different coloring stages[J]. International Journal of Molecular Sciences, 24(16): 12554. |
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