Antibacterial Effect of Bacillus velezensis YFB3-1 on Phytophthora capsici and Its Antibacterial Substances
MU Xiao-Qing1, LU Yao-Xiong1,2,*, DAI Liang-Ying1, HUANG Guo-Lin3, CHEN Wei4, ZHANG Ju-Ye4, WEI Chen-Xi4
1 College of Plant Protection, Hunan Agricultural University, Changsha 410128, China; 2 Institute of Agri-environment and Ecology, Hunan Academy of Agricultural Sciences, Changsha 410125, China; 3 Horticultural Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China; 4 College of horticulture, Hunan Agricultural University, Changsha 410128, China
Abstract Pepper phytophthora blight is a soil-borne disease caused by Phytophthora capsic, which causes devastating losses of pepper (Capsicum annuum) and seriously threatens the sustainable development of pepper industry in China. In order to obtain a safe, efficient and effective biocontrol agent for the prevention of pepper phytophthora blight, a strain of Bacillus velezensis YFB3-1 isolated and identified from the intestinal bacteria of earthworms (Eisenia foetida) was selected as a biocontrol strain, and Phytophthora capsici was used as the target strain. The antibacterial effects of different types of Bacillus on P. capsici were compared by plate confrontation method, and the antibacterial effects of YFB3-1 on different soil-borne pathogenic fungi of pepper were compared, and the main antibacterial secondary metabolites and biocontrol mechanisms were analyzed. The antibacterial test showed that B. velezensis YFB3-1 had the best inhibitory effect on P. capsici, and the inhibition rate was 85.21%, which was 19.8% higher than that of B. subtilis GCK5-1. It had good antibacterial effect on different soil-borne pathogenic fungi of pepper, and had the characteristics of broad-spectrum resistance to soil-borne pathogenic fungi of pepper. Microscopic examination showed that B. velezensis YFB3-1 could lead to abnormal mycelial growth, multi-branching or disordered branches, resulting in distortion and enlargement of mycelial cells to varying degrees, and even cell wall rupture. The results of liquid chromatography tandem mass spectrometry (LC-MS) targeted metabolomics showed that the main lipopeptide compounds in the fermentation broth of B. velezensis YFB3-1 were surfactin and fengycin, which reached the highest content at 3 d of fermentation, which were 1.19×10-3 and 1.14×10-3 mg/mL, respectively.This study provides an excellent biocontrol strain for the green prevention and control of pepper blight, and provides a reference for further excavation and utilization of secondary metabolites of B. velezensis.
MU Xiao-Qing,LU Yao-Xiong,DAI Liang-Ying, et al. Antibacterial Effect of Bacillus velezensis YFB3-1 on Phytophthora capsici and Its Antibacterial Substances[J]. 农业生物技术学报, 2024, 32(4): 859-872.
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