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| Genome-wide Analysis of the Expansin Gene Family in Grape (Vitis vinifera) and Functional Study of VvEXPA27 |
| ZHANG Jian-Peng, SHANG Xiao-Li* |
| Puyang Vocational and Technical College, Puyang 457000, China |
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Abstract The size of berry is one of the important factors determining the appearance quality and yield of grapes (Vitis vinifera), and cell expansion is crucial for determining the final size of berry. Expansin (EXP) is a cell wall relaxation protein, which promotes cell expansion by breaking the noncovalent bond between cellulose microfibers and related matrix polysaccharides in the cell wall. This study used the 'Pinot Noir PN4002' genome as a reference and used HMMER to identify the whole genome of grape expansin family members based on Pfam database. After eliminating redundant sequences, a total of 31 members were screened. Two candidate genes, VvEXPA06 and VvEXPA27, were identified through bioinformatics analysis. The RT-qPCR results showed that VvEXPA06 was only upregulated at 20 d after flowering, while VvEXPA27 was upregulated at both 20 and 48 d after flowering. Subsequently, the VvEXPA27 were subjected to heterologous expression in Arabidopsis thaliana and their transgenic plants were functionally validated. Compared with the vector control (VC), the cell area of the third leaf, silique length, and rosette diameter of T3-generation homozygous transgenic A. thaliana overexpressing (OE) the VvEXPA27 gene showed a significant increase. The results showed that VvEXPA27 played a positive role in cell expansion. This study provides a theoretical basis for analyzing the molecular mechanism of berry cell expansion and genetic resources for molecular breeding.
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Received: 22 July 2023
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Corresponding Authors:
*shangxiaolipy@163.com
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