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| Effects of Conjugated Linoleic Acid on Proliferation and Differentiation of Bovine (Bos taurus) Intramuscular Preadipocyte |
| WAN Rong1,*, NONG Si-Wei1, ZHOU Zhen-Ming2, MENG Qing-Xiang2 |
1 Agriculture and Food Engineering College/Industrial College of Subtropical Characteristic Agriculture, Baise University, Baise 533000, China;
2 Beef Cattle Research Center, China Agricultural University, Beijing 100193, China |
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Abstract The degree of proliferation and differentiation of adipocytes deposited in muscle fibers or between muscle bundles determines the level of beef marbling index directly, which affects the quality level of beef. This study was to assess the effects of conjugated linoleic acid (CLA) on the proliferation and differentiation of intramuscular preadipocytes in Luxi cattle (Bos taurus). In vitro cell model was developed for proliferation of bovine intramuscular preadipocytes which were cultured in growth medium supplemented with CLA (50, 100 and 150 μmol/L) for 4 d. 100 μmol/L CLA significantly increased the proportion of S phase cells (P<0.05, increased by 1.63 times), decreased the proportion of G0/G1 phase cells (P<0.05, decreased by 9.2%), and the other treatment groups had no significant difference (P>0.05). The results showed that 100 μmol/L CLA significantly increased the proliferation rate of bovine intramuscular preadipocyte by flow cytometry (P<0.05). Bovine intramuscular preadipocytes were cultured in differentiation media with different doses of CLA, the induction of adipogenesis were indicated by measuring the lipid droplets. Six days later, lots of lipid droplets were observed in differentiated preadipocytes, in which cytosolic lipid content remarkably increased at 100 and 150 µmol/L CLA, respectively (P<0.05). Four combination of CLA (100 µmol/L), insulin (10 μg/mL) and dexamethasone (0.25 μg/mL) was designed and cultured cells were induced for 6 d with CLA, insulin and dexamethasone. When dexamethasone, insulin or CLA were removed, fat content in the cytoplasm decreased by 74%, 68% and 31%, respectively. The combination of CLA, insulin, and dexamethasone also resulted in the greatest cytosolic lipid content. 100 μmol/L CLA can increase the expression of peroxisome proliferator-activated receptor-γ (PPARγ) gene by about 3 times, and the expression level of CCAAT/enhancer-binding protein-α (C/EBPα) gene by about 2 times (P<0.05). Taken together, the present findings indicated that CLA may promote bovine intramuscular preadipocyte proliferation and differentiation, which highlighted the potential use of CLA on bovine intramuscular preadipocyte proliferation and differentiation to improve beef quality.
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Received: 23 November 2022
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Corresponding Authors:
* qingfengyimeng118@126.com
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[1] 陈银基, 周光宏, 邹晓葵, 等. 2007. 肉牛胴体背最长肌脂肪酸组成与大理石花纹等级相关性研究[J]. 中国农业科学, 40(12): 2853-2860.
(Chen Y J, Zhou G H, Zou X K, et al.2007. Research on relationship between beef marbling standard and fatty acid composition of longissimus dorsi[J]. Scientia Agricultura Sinica, 40(12): 2853-2860.)
[2] 蒋常文, 闫蕴力, 马卫东, 等. 2006. 外源性IL-18基因对C6胶质瘤细胞周期及相关基因表达的影响[J]. 肿瘤, 26(5): 436-439.
(Jiang C W, Yan Y L, Ma W D, et al.2006.Effects of exogenous IL-18 gene transfection on cell cycle and related gene expression of C6 glioma cells[J]. Tumor, 26(5): 436-439.)
[3] 万荣, 周振明, 孟庆翔. 2021. 丙酸对肉牛肌内前脂肪细胞分化的影响[J]. 农业生物技术学报, 29(7): 1295-1302.
(Wan R, Zhou Z M, Meng Q X.2021. Effects of propionate on differentiation of bovine (Bos taurus) intramuscular preadipocytes[J]. Journal of Agricultural Biotechnology, 29(7): 1295-1302.)
[4] 邹朝霞, 李影. 2021. 共轭亚油酸减少动物脂肪沉积的分子机制研究进展[J]. 中国细胞生物学学报, 43(10): 2061-2070.
(Zou Z X, Li Y.2021. Advances in the molecular mechanism of conjugated linoleic acids in decreasing fat deposition[J]. Chinese Journal of Cell Biology, 43(10): 2061-2070.)
[5] Braissant O, Foufelle F, Scotto C.1996. Differential expression of peroxisome proliferator-activated receptor (PPAR): Tissue distribution of PPAR-α, -β and -γ in the adult rat[J]. Endocrinology, 137(1): 354-366.
[6] Christy R J, Kaestner K H, Geiman D E, et al.1991. CCAAT/enhancer binding protein gene promoter: Binding of nuclear factors during differentiation of 3T3-L1 preadipocytes[J]. Proceedings of the National Academy of Sciences of the USA, 88(6): 2593-2597.
[7] Chung K Y, Smith S B, Choi S H, et al.2016. Oleic acid enhances G protein coupled receptor 43 expression in bovine intramuscular adipocytes but not in subcutaneous adipocytes[J]. Journal of Animal Science, 94(5): 1875-1883.
[8] Devatkal S, Kalpana S, Reddy P B.2018. Estimation of conjugated linoleic acid (CLA) content in ruminant meat and fats[J]. Meat Science, 13(2): 30-33.
[9] Evans M, Park Y, Pariza M, et al.2001. Tran-10, cis-12 conjugated linoleic acid reduces triglyceride content while differentially affecting PPARγ2 and ap2 expression in 3T3-11 preadipocytes[J]. Lipids, 36(11): 1223-1232.
[10] Ha Y L, Grimm N K, Pariza M W.1987. Anticarcinogens from fried ground beef: Heat-altered derivatives of linoleic acid[J]. Carcinogenesis, 8(12): 1881-1887.
[11] Hartwell L H, Weinert T A.1989. Checkpoints: Controls that ensure the order of cell cycle events[J]. Science, 246(4930): 629-634.
[12] Hausman G J, Bergen W G, Etherton T D, et al.2018. The history of adipocyte and adipose tissue research in meat animals[J]. Journal of Animal Science, 96(2): 473-486.
[13] Jack E L, Hamm J K, Pilch P F, et al.1999. Reconstitution of insulin-sensitive glucose transport in fibroblast requires expression of both PPARg and C/EBPα[J]. Journal of Biological Chemistry, 274(12): 7946-7951.
[14] Jiang T, Shi X, Yan Z, et al.2019. Isoimperatorin enhances 3T3-L1 preadipoycte differentiation by regulating PPARγ and C/EBPα through the Akt signaling pathway[J]. Experimental and Therapeutic Medicine, 18(3): 2160-2166.
[15] Jiang Z Y, Zhong W J, Zheng C T, et al.2010. Conjugated linoleic acid differentially regulates fat deposition in backfat and longissimus muscle of finishing pigs[J]. Journal of Animal Science, 88(5): 1694-1705.
[16] Kim J, Chung K, Fuerniss L K, et al.2020. Oleic acid stimulates the formation of adipocyte-like cells from bovine satellite cells via G-protein coupled receptor 43 and CCAT/enhancer-binding protein beta[J]. Open Journal of Animal Sciences, 10(4): 649-664.
[17] Kirkland J L, Hollenberg C H, Gillon W S.1996. Effects of fat depot site on differentiation-dependent gene expression in rat preadipocytes[J]. The International Journal of Obesity, 20(Suppl. 3): S102-S107.
[18] Lin W, Chen L, Meng W, et al.2022. C/EBPα promotes porcine pre-adipocyte proliferation and differentiation via mediating MSTRG.12568.2/FOXO3 trans-activation for STYX[J]. Biochimica et Biophysica Acta-molecular and Cell Biology of Lipids, 1867(10): 159206.
[19] Listrat A, Lebret B, Louveau I, et al.2016. How muscle structure and composition influence meat and flesh quality[J]. The Scientific World Journal, 2016: 1-14.
[20] McNeel R L, Mersmann H J.2003. Effects of isomers of conjugated linoleic acid on porcine adipocyte growth and differentiation[J]. Journal of Nutritional Biochemistry, 14(5): 266-274.
[21] Pu Y, Veiga-Lopez A.2017. PPARγ agonist through the terminal differentiation phase is essential for adipogenic differentiation of fetal ovine preadipocytes[J]. Cellular & Molecular Biology Letters, 22(6): 1-12.
[22] Ramirez-Zacarias J L, Castro-Munozledo F, Kuri-Harcuch W.1992. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with oil red O[J]. Histochemistry, 97(6): 493-497.
[23] Ramsay T G, Rosebrough R W.2003. Hormonal regulation of postnatal chicken preadipocyte differentiation in vitro[J]. Comparative Biochemistry and Physiology B, 136(2): 245-253.
[24] Schmidt S E, Thelen K M, Raphael W, et al.2016. Effects of trans-10, cis-12 conjugated linoleic acid on gene expression and lipid content of adiocytes derived from lactating dairy cows[J]. Journal of Animal Science, 94(5): 370-371.
[25] Spiegelman B.1998. PPARγ: Adipogenic regulator and thiazolidinedione receptor[J]. Diabetes, 47(4): 507-514.
[26] Umek R, Freidman A, McKnight S.1991. CCAAT-enhancer binding protein: A component of a differentiation switch[J]. Science, 251(4991): 288-292.
[27] Wabitsch M, Heinze E, Hauner H, et al.1996. Biological effects of human growth hormone in rat adipocyte precursor cells and newly differentiated adipocytes in primary culture[J]. Metabolism, 45(1): 34-42.
[28] Wan R, Du J P, Ren L P, et al.2009. Selective adipogenic effects of propionate on bovine intramuscular and subcutaneous preadipocytes[J]. Meat Science, 82(3): 372-378.
[29] Yeganeh A, Taylor C G, Tworek L, et al.2016. Trans-10, cis-12 conjugated linoleic acid (CLA) interferes with lipid droplet accumulation during 3T3-L1 preadipocyte differentiation[J]. The International Journal of Biochemistry & Cell Biology, 76(7): 39-50.
[30] Yeganeh A, Zahradka P, Taylor C G.2017. Trans-10, cis-12 conjugated linoleic acid (t10-c12 CLA) treatment and caloric restriction differentially affect adipocyte cell turnover in obese and lean mice[J]. Journal of Nutritional Biochemistry, 49(11): 123-132.
[31] Yeh W C, Cao Z, Classon M.1995. Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP family of leucine zipper proteins[J]. Genes & Devolopment, 9(2): 168-181.
[32] Yu Y, Correll P, Heuvel J P.2002. Conjugated linoleic acid decreases production of pro-inflammatory products in macrophages: Evidence for a PPAR gamma-dependent mechanism[J]. Biochimica et Biophysica Acta, 1581(3): 89-99.
[33] Zhou X, Li D F, Yin J D, et al.2007. CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle[J]. Journal of Lipid Research, 48(8): 1701-1709. |
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