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农业生物技术学报  2024, Vol. 32 Issue (1): 189-199    DOI: 10.3969/j.issn.1674-7968.2024.01.017
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Inhibitory Effect of PEX10 Gene Deletion on the Growth and Pathogenicity of Trichophyton mentagrophytes in Oryctolagus cuniculus
PAN Yao1,2, XIAO Chen-Wen2, LIU Yan2, ZHANG Peng-Jun1, YING Shu-Min3, HUANG Ye-E2, JI Quan-An2, WEI Qiang2, WANG Jing3, WANG Jao-Yu3,*, BAO Guo-Lian2,*
1 College of Life Sciences, China Jiliang University, Hangzhou 310018, China;
2 Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3 Institute of Plant Protection and Microbiology Science, Zhejiang Academy of Agricultural Sciences/The National Key Laboratory for Agricultural Product Quality and Safety Hazard Factors and Risk Prevention and Control, Hangzhou 310021, China
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Abstract  Trichophyton mentagrophytes, also known as T. gypsioides, is severely hazardousness to rabbit (Oryctolagus cuniculus). Peroxisomal biogenesis factor 10 (PEX10) gene is closely related to the drug susceptibility of plant pathogenic fungi. However, the mechanism of whether PEX10 gene affects the growth and pathogenicity of animal pathogenic fungi such as T. mentagrophytes is still unclear. This study first constructed a PEX10 gene knockout vector and obtained a PEX10 gene knockout strain of T. mentagrophytes through Agrobacterium tumefaciens-mediated transformation (AtMT) and transformant screening. Phenotypic analysis such as growth characteristics showed that the growth rate of PEX10 gene knockout strains was significantly slowed down (P<0.01), and their sensitivity to berberine hydrochloride was significantly increased (P<0.001). The virulence and pathogenicity of the PEX10 knockout strain to rabbit skin was reduced. The results of this study provide reference for the study of PEX10 gene function and the prevention and treatment of T. mentagrophytes of rabbits.
Key wordsTrichophyton mentagrophytes      Peroxisomal biogenesis factor 10 (PEX10)      Perxisome      Drug resistance     
Received: 13 December 2022     
ZTFLH:  S182  
  S85  
Corresponding Authors: * Corresponding authors, baoguolian@163.com; wangjiaoyu78@sina.com   
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Articles by authors
PAN Yao
XIAO Chen-Wen
LIU Yan
ZHANG Peng-Jun
YING Shu-Min
HUANG Ye-E
JI Quan-An
WEI Qiang
WANG Jing
WANG Jao-Yu
BAO Guo-Lian
Cite this article:   
PAN Yao,XIAO Chen-Wen,LIU Yan, et al. Inhibitory Effect of PEX10 Gene Deletion on the Growth and Pathogenicity of Trichophyton mentagrophytes in Oryctolagus cuniculus[J]. 农业生物技术学报, 2024, 32(1): 189-199.
URL:  
https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2024.01.017     OR     https://journal05.magtech.org.cn/Jwk_ny/EN/Y2024/V32/I1/189
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