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| Inhibitory Effect of PEX10 Gene Deletion on the Growth and Pathogenicity of Trichophyton mentagrophytes in Oryctolagus cuniculus |
| PAN Yao1,2, XIAO Chen-Wen2, LIU Yan2, ZHANG Peng-Jun1, YING Shu-Min3, HUANG Ye-E2, JI Quan-An2, WEI Qiang2, WANG Jing3, WANG Jao-Yu3,*, BAO Guo-Lian2,* |
1 College of Life Sciences, China Jiliang University, Hangzhou 310018, China;
2 Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3 Institute of Plant Protection and Microbiology Science, Zhejiang Academy of Agricultural Sciences/The National Key Laboratory for Agricultural Product Quality and Safety Hazard Factors and Risk Prevention and Control, Hangzhou 310021, China |
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Abstract Trichophyton mentagrophytes, also known as T. gypsioides, is severely hazardousness to rabbit (Oryctolagus cuniculus). Peroxisomal biogenesis factor 10 (PEX10) gene is closely related to the drug susceptibility of plant pathogenic fungi. However, the mechanism of whether PEX10 gene affects the growth and pathogenicity of animal pathogenic fungi such as T. mentagrophytes is still unclear. This study first constructed a PEX10 gene knockout vector and obtained a PEX10 gene knockout strain of T. mentagrophytes through Agrobacterium tumefaciens-mediated transformation (AtMT) and transformant screening. Phenotypic analysis such as growth characteristics showed that the growth rate of PEX10 gene knockout strains was significantly slowed down (P<0.01), and their sensitivity to berberine hydrochloride was significantly increased (P<0.001). The virulence and pathogenicity of the PEX10 knockout strain to rabbit skin was reduced. The results of this study provide reference for the study of PEX10 gene function and the prevention and treatment of T. mentagrophytes of rabbits.
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Received: 13 December 2022
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Corresponding Authors:
* Corresponding authors, baoguolian@163.com; wangjiaoyu78@sina.com
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