Expression Analysis of the StNF-YA8 Gene Associated with Dormancy Release in Potato (Solanum tuberosum)
WANG Xiao1,2, WEI Han1, LI Shi-Gui1, WANG Kai-Tong1,2, ZHAO Liang3, ZHANG Ning1,2, SI Huai-Jun1,2,*
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; 3 Qitai Wheat Experimental Station, Xinjiang Academy of Agricultural Sciences, Qitai 831800, China
Abstract Nuclear factor-Y (NF-Y) is a class of transcription factors widely distributed in eukaryotes. It composed of NF-YA, NF-YB and NF-YC subunits. NF-YA is involved in regulating plant growth and development, and stress response. In this study, qRT-PCR method was used to analyze the expression pattern of 10 StNF-YA genes of potato (Solanum tuberosum) in the process of tuber dormancy release. The results showed that the StNF-YA8 gene was consistently up-regulated in expression during dormancy release in tubers. The StNF-YA8 gene was further cloned (GenBank No. XM_015309982.1), and its ORF was 591 bp, encoding 196 amino acids. The subcellular localization vector of StNF-YA8 protein was constructed, and the results showed that StNF-YA8 protein was localized in the nucleus and cell membrane. Tissue-specific analysis revealed that the StNF-YA8 gene was differentially expressed between different tissues. The results of bioinformatics analysis showed that the potato StNF-YA8 protein contained a conserved CBFB domain, and had the highest evolutionary relationship with wild potato (S. commersonii); StNF-YA8 protein closely interacted with StNF-YB3/5/10/14/17 and StNF-YC1/4/5/8/9 proteins; there were cis-acting elements involved in light response, circadian rhythm control and hormone response in the promoter sequence of this gene. The results can provide a basis for the molecular mechanism analysis of potato StNF-YA8 gene involved in regulating tuber dormancy release.
WANG Xiao,WEI Han,LI Shi-Gui, et al. Expression Analysis of the StNF-YA8 Gene Associated with Dormancy Release in Potato (Solanum tuberosum)[J]. 农业生物技术学报, 2024, 32(1): 50-59.
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