Identification of Bacillus amyloliquefaciens M1-1 and Its Antifungal and Plant Growth Promoting Effects
WANG Shi-Wei1,*, WANG Qing-Hui2
1 College of Life Sciences and Agriculture and Forestry/Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, Qiqihar University, Qiqihar 161006, China; 2 School of Life Science, Northeast Agricultural University, Harbin 150030, China
Abstract Baijiu Daqu contains rich microorganisms. Among them, Bacillus amyloliquefaciens can inhibit plant pathogens, be used in biological control, promote plant growth, and have potential application value in agricultural production. In this study, LB medium was used to isolate the B. amyloliquefaciens from Beidacang Baijiu Daqu, and the strain were identified by the morphology and molecular biology such as colony, cell observation, spore staining, 16S rRNA gene sequencing and comparison techniques. This bacterium was the most similar to Bacillus amyloliquefaciens (GenBank No. MG593943.1) with 100% consistency and 95% coverage. It belonged to B. amyloliquefaciens and was named as M1-1 strain. It was submitted to the NCBI website and obtained the GenBank No. OM321552. The inhibitory effects of B. amyloliquefaciens M1-1 antibacterial protein on 4 plant pathogenic fungi, namely Fusarium oxysporum M1, Phacidiopycnis washingtonensis, Gibberella and Rhizoctonia solani, were studied by plate confrontation and Oxford cup methods, respectively. The fermentation broth of B. amyloliquefaciens M1-1 had strong antibacterial effects on all 4 plant pathogens mentioned above, with a antifungal rate ranging from 43% to 61%; In addition to producing protease, B. amyloliquefaciens M1-1 could also produce a variety of antifungal proteins, and the diameter of the inhibitory ring was between 1.8 and 3.0 cm; The pot experiment method was used to study the promoting effect of B. amyloliquefaciens M1-1 on the growth of Bryophyllum pinnatum. The experimental results showed that B. amyloliquefaciens M1-1 had a significant promoting effect on the growth of plants. In summary, this B. amyloliquefaciens M1-1 has good inhibitory effects on various plant pathogens and could be applied to the development and utilization of biocontrol agents, and it also has potential application value in increasing crop yield.
WANG Shi-Wei,WANG Qing-Hui. Identification of Bacillus amyloliquefaciens M1-1 and Its Antifungal and Plant Growth Promoting Effects[J]. 农业生物技术学报, 2023, 31(12): 2612-2622.
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