Genome Wide Association Study for Chlorate Resistance in Maize (Zea mays)
WANG Zi-Hui, JI Wei-Dong, WEI Jie, WANG Yun-Yun, WANG Hou-Miao, YANG Ze-Feng, XU Chen-Wu, LI Peng-Cheng*
Agricultural College of Yangzhou University/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou 225009, China
Abstract Chlorate resistance is one of the reliable characters to evaluate crop nitrogen efficiency. To identify QTL and candidate genes for chlorate resistance in maize (Zea mays), the narutal variation of 7 traits were evaluated under control and chlorate treatment in 283 inbred lines. Chlorate resistance was calculated for each traits. The results showed that chlorophyll relative content, plant height, leaf length and shoot dry weight were significantly reduced after chlorate treatment. A total of 133 SNPs were identified by genome-wide association analysis, and 51, 53 and 29 SNPs were identified under normal condition, chlorate treatment and chlorate resistance for each trait. In combined genome wide association study (GWAS) and RNA-seq 13, possible candidate genes were identfied, including cleavage and polyadenylation specificity factor, N-acetylserotonin O-methyltransferase and MYB transcription factor. These results provide candidate genes for understanding the genetic mechanism of chlorate resistance in maize, and could help to identify key gene of nitrogen use efficiency and improve of nitrogen use efficiency in maize.
WANG Zi-Hui,JI Wei-Dong,WEI Jie, et al. Genome Wide Association Study for Chlorate Resistance in Maize (Zea mays)[J]. 农业生物技术学报, 2023, 31(12): 2443-2453.
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