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| Effects of Different Foliar Fertilizer on Physiological Characteristics and Expression of Yield-related Genes of 'Longjing Youdizhan' (Oryza sativa) |
| PENG Jia-Yuan1,*, LIU Jun2,*, LIU Wen-Xiang3,**, ZHANG Zhen-Qian1,** |
1 College of Agronomy, Hunan Agricultural University, Changsha 410128, China;
2 Changde Academy of Agriculture and Forestry Sciences, Changde 515000, China;
3 Hunan Agricultural Information and Engineering Research Institute, Changsha 410125, China |
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Abstract High quality hybrid rice has good quality, loved by the vast number of consumers. Foliar fertilizer, as a way of applying fertilizer outside the roots of crops, has an important effect on the increase of crop yield. In order to screen out a reasonable ratio of foliar fertilizer and improve the yield of high quality rice, 'Longjing Youdizhan' (Oryza sativa) was used in this study. The effects of different foliar fertilizer ratios on the physiological indexes of high-quality hybrid rice leaves, the expression of yield-related genes enoyl-CoA hydratase (NOG1) and pale green leaf-9 (PGL-9) and yield were studied. The combination of brassinolide (BR), silicon (Si) and selenium (Se) foliar fertilizers were used to study 8 different treatments to find out the suitable foliar fertilizer combination based on the yield at harvest. The results showed that the yield of each treatment was higher than that of the control (CK), and the yield of Se treatment was the highest (8.8%), followed by BR treatment (8.3%) and Se+BR treatment (8.2%). Under Si treatment, chlorophyll content and catalase (CAT) activity were the highest at booting stage. Under the treatments of Si+BR, BR and Si, the soluble sugar content, superoxide dismutase (SOD) and peroxidase (POD) activities were the highest at heading stage. Under BR treatment, malonaldehyde (MDA) was the lowest at tillering stage. Correlation analysis showed that the expression of NOG1 and PGL-9 gene and chlorophyll content were significantly correlated with yield at heading stage. The correlation coefficients were -0.881, 0.932 and -0.940, respectively. In addition, the expression of NOG1 and PGL-9 gene were significantly correlated with chlorophyll content at heading stage, and the correlation coefficients were 0.993 and -0.971, respectively. In summary, selenium fertilizer was the best foliar fertilizer, and its application at heading stage can significantly increase the yield of high-quality rice 'Longjing Youdizhan'. This study can provide reference for high-yield cultivation techniques of high-quality rice.
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Received: 15 June 2022
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Corresponding Authors:
** hnaieilwx163@hunaas.cn; zzq770204@163.com
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| About author:: * These authors contributed equally to this work |
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[1] 郭荟, 屠乃美, 马国柱, 等. 2021. 硅肥对双季水稻生长发育及产量的影响[J]. 作物研究, 35(06): 549-554.
(Guo H, Tu N M, Ma G Z, et al.2021. Effects of silicon fertilizer on growth and yield of double cropping rice[J]. Crop Research, 35(06): 549-554.)
[2] 郭美俊, 郭平毅, 原向阳. 2014. 叶面喷施亚硒酸钠对谷子光合特性及产量构成的影响[J]. 核农学报, 28(06): 1099-1107.
(Guo M J, Guo P Y, Yuan X Y.2014. Effects of foliar spraying of sodium selenite on photosynthetic characteristics and yield components of Foxtail mille[J]. Journal of Nuclear Agricultural Sciences, 28(06): 1099-1107.)
[3] 郭天宇, 徐宁彤, 曲琪环. 2017. 叶面喷施不同硒肥对水稻含硒量及产量的影响[J]. 江苏农业科学, 45(07): 59-61.
(Guo T Y, Xu N T, Qu Q H.Effects of foliar spraying different Selenium fertilizer on selenium content and yield of rice[J]. Jiangsu Agricultural Sciences, 2017, 45(07): 59-61.)
[4] 胡萍. 2020. 水稻光合相关基因PGL-9的鉴定和克隆[D]. 硕士学位论文, 中国农业科学院, 导师: 郭龙彪, pp. 1-60.
(Hu P.2020. Identification and cloning of photosynthetic related gene PGL-9 in rice[D]. Thesis for M.S., Chinese Academy of Agricultural Sciences, Supervisor: Guo L B. pp. 1-60.)
[5] 霍兴. 2018. 水稻产量基因NOG1的克隆与功能分析[D]. 博士学位论文, 中国农业大学, 导师: 孙传清. pp. 1-97.
(Huo X.Cloning and functional analysis of rice yield gene NOG1[D]. Thesis for Ph.D., China Agricultural University, Supervisor: Sun C Q, pp. 1-97.)
[6] 李小明, 龙惊惊, 周悦, 等. 2017. 叶面肥的应用及研究进展[J]. 安徽农业科学, 45(03): 127-130.
(Li X M, Long J J, Zhou Y, et al.2017. Application and research progress of foliar fertilizer[J]. Anhui Agricultural Sciences, 45(03): 127-130.)
[7] 廖西元, 陈庆根, 庞乾林. 2002. 我国优质水稻生产现状与发展对策[J]. 农业技术经济, (05): 32-34.
(Liao X Y, Chen Q G, Pang Q L, et al. 2002. Production status and development countermeasures of high quality rice in China[J]. Agrotechnical Economy, (05): 32-34.)
[8] 刘信, 刘春青, 王玉玺, 等. 2022. 我国优质稻品牌化发展现状及建议[J]. 中国稻米, 28(02): 12-15.
(Liu X, Liu C Q, Wang Y X, et al.2022. Development status and Suggestions of high quality rice brand in China[J]. The Chinese Rice, 28(02): 12-15.)
[9] 马跃峰, 郭成林, 杜晓莉, 等. 2008. 油菜素内酯对直播稻素质的影响及增产效果[J]. 农药,(11):845-846, 856.
(Ma Y F, Guo C L, Du X L, et al.Effects of brassinolide on quality of direct-seeding rice and yield increase effect[J]. Agrochemicals,(11): 845-846, 856.)
[10] 欧阳杰, 王楚桃, 何光华, 等. 2012. 水稻灌浆中后期功能叶中叶绿素含量及其变化趋势与谷物产量关系研究[J]. 西南农业学报, 25(04): 1201-1204.
(Ou Y J, Wang C T, He G H, et al.2012. Study on the relationship between grain yield and chlorophyll content in functional leaves of rice during middle and late filling period[J]. Southwest China Journal of Agriculture, 25(04): 1201-1204. )
[11] 王吉生. 2011. 孕穗期施用2,4-表油菜素内酯对水稻籽粒灌浆及产量的影响[D]. 硕士学位论文, 四川农业大学, 导师: 王西瑶. pp. 1-36.
(Wang J S.2011. Effects of 2,4-epbrassinolide application at booting stage on grain filling and yield of rice [D]. Thesis for M.S., Sichuan Agricultural University , Supervisor: Wang X Y. pp. 1-36.)
[12] 王晓丹, 肖钢, 张振乾, 等. 2019. 高油酸油菜脂肪酸代谢相关微效基因筛选及验证[J]. 农业生物技术学报, 27(07): 1171-1178.
(Wang X D, Xiao G, Zhang Z Q, et al.2019. Screening and verification of minor genes related to fatty acid metabolism with high oleic acid[J]. Journal of Agricultural Biotechnology, 27(07): 1171-1178.)
[13] 萧浪涛, 王三根. 2005. 植物生理学试验技术[M]. 中国农业出版社, 北京. pp. 211-215.
(Xiao L T, Wang S G.2005. Experimental technology of plant physiology[M]. China Agriculture Press, Beijing. pp. 211-215.)
[14] 许祥富, 林明义. 2021. 我国新型肥料的研究现状及在水稻上的应用进展[J]. 安徽农业科学, 49(07): 17-19, 29.
(Xu X F, Lin M Y.2021. Research status of new fertilizer and its application in rice in China[J]. Anhui Agricultural Sciences, 49(07): 17-19, 29.)
[15] 杨国英, 郭智, 盛婧, 等. 2021. 不同生育时期施用硅肥对优质食味粳稻产量和品质的影响[J]. 中国稻米, 27(01): 68-74, 79.
(Yang G Y,Guo Z, Sheng J, et al.2021. Effects of silicon fertilizer application at different growth stages on yield and quality of japonica rice with good taste[J]. The Chinese Rice, 27(01): 68-74, 79.)
[16] 杨艳君, 张谨华, 冀瑞萍, 等. 2015. 不同密度、油菜素内酯和 2,4-D丁酯对谷子光合特性及产量的影响[J]. 作物杂志, ( 6): 84-90.
(Yang Y J, Zhang J H, Yi D P, et al. 2015. Effects of different density, brassinolide and 2, 4-D butyl ester on photosynthetic characteristics and yield of Millet[J]. Crops, ( 6): 84-90.)
[17] 赵飞, 刘建, 杜锦, 等. 2021. 硒肥处理对水稻产量构成因素和产量的影响[J]. 北方水稻, 51(03): 8-10.
(Zhao F, Liu J, Du J, et al.2021. Effects of Selenium fertilizer treatment on yield components and yield of rice[J]. The Northern Rice, 51(03): 8-10.)
[18] 曾波, 钟育海, 郭利磊, 等. 2019. 我国优质水稻品种发展现状与展望[J]. 种子, 38(08): 53-56.
(Zeng B, Zhong Y H, Guo L L, et al.2019. Development status and prospect of high quality rice varieties in China[J]. Seed, 38(08): 53-56.)
[19] Nanthana C, Benjavan R, Tonapha P, et al.2020. Silicon application improves caryopsis development and yield in rice[J]. Journal of the Science of Food and Agriculture, 101(1): 220-228.
[20] Pati S, Pal B, Badole S, et al.2016. Effect of silicon fertilization on growth, yield, and nutrient uptake of rice[J]. Communications in Soil Science and Plant Analysis, 47(3): 284-290. |
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