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| Expression Patterns Analysis of GATA Transcription Factors in Nitrogen Source Response in Setosphaeria turcica |
| WANG Jian-Xia*, WANG Qing*, ZHAO Yu-Lan, LI Tian-Cong, LONG Feng, ZHU Hang, SHEN Shen**, HAO Zhi-Min** |
| Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Science, Hebei Agricultural University, Baoding 071000, China |
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Abstract The GATA transcription factor family contains a zinc finger protein conserved domain that recognizes and binds the 5'-(A/T) GATA (A/G)-3' motif to regulate the transcription and expression of multiple functional genes in eukaryotes. It is closely related to the growth and development of pathogenic fungi and the utilization of nitrogen sources. In order to explore the function of the GATA transcription factor family of Setosphaeria turcica, the Softberry database (http://linux1.softberry.com/berry.phtm) was employed to carry out genetic structure analysis, prediction of conserved domains and promoters; qRT-PCR was used to detect the effects of nitrogen source concentration levels and species on gene transcription levels of family members. The results showed that there were 7 members in the GATA transcription factor family of S. turcica. Compared with KNO3 as the nitrogen source, in the medium with NH4Cl as the only nitrogen source, the growth rate of hyphae was accelerated and the conidia production was reduced by about 15 times, and the transcription levels of all GATA transcription factors were increased by 2 to 4 times. Compared with the normal Richard's medium, when KNO3 content was 0, the growth rate of hyphae was increased and the yield of conidia was reduced by about 10 times. At the same time, the expression of all GATA transcription factors was increased by 20 to 40 times, indicating that GATA transcription factor expressions was highly induced under the completely restricted nitrogen source. The above results preliminarily clarified the response of GATA transcription factors to nitrogen metabolism, revealed the availability of nitrogen and the regulatory role of GATA transcription factors in the development of S. turcica, and provide new ideas for the control of northern corn leaf blight.
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Received: 23 December 2019
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Corresponding Authors:
** shenhsen0428@163.com; hzm_0322@163.com
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| About author:: * The authors who contribute equally |
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