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| Segregation Distortion Analysis on Maize (Zea mays) RIL Population by SNP |
| FU Zhong-Jun, GUAN Ling, ZHANG Pi-Hui, DONG Xin, LI Shu-Jun, CHEN Rong-Li, QI Zhi-Yun*, YANG Hua* |
| Chongqing Academy of Agricultural Sciences, Chongqing 401329, China |
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Abstract Segregation distortion is an ubiquitous phenomenon in nature and an important driving force for biological evolution. With the development and progress of molecular marker technology, a large number of segregation distortion phenomena are reported in the genetic research of different crops and the proportion of marker segregation distortion and genetic effect vary according to species, population, marker type, etc., while there were segregation distortion regions (SDRs). In order to investigate the phenomenon of maize (Zea mays) segregation distortion and its causes, a F8 population consisting of 198 recombinant inbred lines (RIL) was used in the study, which derived from a cross between a tropical maize inbred line B31-3 and a temperate maize inbred line HZ4. A total of 747 SNPs, covering 10 maize chromosomes, were detected with polymorphism between parent B31-3 and HZ4. Segregation distortion analysis was performed among the 747 SNPs for the RIL population. The results of chi-square test (χ2 =96.92, >χ20.05=6.63)(P<0.05), showed that the distribution proportion of the female B31-3 and male HZ4 genotypes deviated from the theoretical ratio of 1∶1 in RIL population and the genetic contribution rate of female B31-3 to offspring was higher than that of male HZ4. The result indicated that 279 SNPs (37.3%) showed the genetic distortion (P<0.05). Of the total segregation distortion SNPs, 194 SNPs (69.5%) deviated toward female parent B31-3, while 85 SNPs (30.5%) deviated toward male parent HZ4. Totally, 10 SDRs were detected among 6 chromosomes. 3 SDRs were distorted to HZ4, while 7 SDRs were skewed to B31-3, which showed female parent gametophytes had an competitive advantage in forming a zygote in this study. There were 6~64 segregation distortion SNPs in SDRs and the span of SDRs were 3.69~78.11 cM. SDR5-1 and SDR5-3, detected in this study, were located in near regions where gametophyte genes related to segregation distortion had been reported. Additionally, in the study, the RIL population was affected by high temperature in the group building process. So, the occurrence of segregation distortion and the formation of SDRs might be related to gametic selection and high temperature environment in this study. This study provides basic data for understanding the law of segregation distortion of maize.
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Received: 14 January 2019
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Corresponding Authors:
qzy5609@163.com;cqyangh@163.com
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