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| Cloning and Drought Resistance Identification of TaXTH-7A Gene in Wheat（Triticum aestivum） |
| 琪 周,燕茹 冯,Li Song, |
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Abstract Wheat is one of the important food crops in China. However, drought and other adverse conditions have seriously affected the quality and yield of wheat. Screened the stress-resistant genes, and analyzed mechanismes of action, providing candidate genes and theoretical basis for wheat resistance breeding. Xyloglucan endotransglucosylase/hydrolase (XET/XEH) is a class of enzymes with xyloglucan endoglycosyltransferase /hydrolase activity, which are collectively referred to as XTH, belonging to glycoside hydrolase 16 family. It can participate in the formation and remodeling of cell wall and plays an important role in plant growth and development and stress resistance. In this study, the cDNA sequence of the TaXTH-7A gene was isolated by PCR from wheat (Triticum aestivum L. ), the coding region of it was 870 bp in length, encoding 290 amino acid residues, and its genome contained 3 exons and 2 introns. Protein sequence analysis indicated that TaXTH-7A contains several XET-specific domains and they are highly conserved across species. Phylogenetic tree analysis indicated that the XTH-7AL gene evolved between monocotyledonous and dicotyledonous, and between C3 and C4 plants, and was closely related to Aegilops tauschii. The results of qRT-PCR showed that TaXTH-7A gene was expressed in roots, stems, leaves and spiket of wheat, and it was dominant in roots. After drought stress, its expression increased and peaked at 4 hours. Subcellular localization results showed that TaXTH-7A is localized in the cytoplasm, cell membrane, and apoplast.It was also found that TaXTH-7A increased the drought resistance of transgenic Arabidopsis. This study provides a basis for further exploration of the function of TaXTH-7A gene in wheat, and also provides a theoretical basis for wheat resistance to abiotic stress.
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Received: 06 March 2019
Published: 03 June 2019
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Corresponding Authors:
琪 周
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