Study on the Inhibitory Effect of Rhizosphere Biocontrol Bacteria on Fusarium oxysporum in Tomato (Solanum lycopersicum) Grafted Seedlings
GAO Xing1,*, CHEN Li-Da1,2,*, CHAI A-Li2, XIE Xue-Wen2, GAO Wei3, BEN Hai-Yan3, WU Xiao-Lei1, GONG Bin-Bin1, LYU Gui-Yun1, WU Jun4, LI Bao-Ju2,**, GAO Hong-Bo1,**
1 College of Horticulture, Hebei Agricultural University, Baoding 071001, China; 2 Insititute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Plant Protection Research Institute, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China; 4 Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Abstract：Grafting, as an important means of preventing soil borne diseases and overcoming continuous cropping obstacles, has been widely used in tomato (Solanum lycopersicum) production. To investigate the interaction between the resistance of grafted tomatoes to wilt disease and beneficial microorganisms in the rhizosphere soil. In this study, the tomato rootstock 'TMS' and scion 'Huangxing No. 1' were used for grafting. The dilution plate method was used to isolate and screen beneficial microorganisms in the tomato grafted rhizosphere, and the biological characteristics and indoor antibacterial activity of beneficial microorganisms were determined. The results showed that the number of cultivable bacteria and biocontrol bacteria in tomato grafted rhizosphere soil was significantly higher than that in seedlings (P<0.05). Using the plate confrontation method, Bacillus subtilis SF61 and Bacillus velezensis SF105 were successfully screened from 128 strains of biocontrol bacteria, showing antibacterial effects against Fusarium oxysporum with inhibition rates of 63.53% and 69.41%, respectively. In addition, strains SF61 and SF105 both had the ability to produce siderophore and amylase (without potassium and phosphorus solubilization functions), and significantly reduced glucosidase and unit pigment content (P<0.05). Among them, the siderophore produced by SF61 and SF105 had inhibitory effects on the hyphae of F. oxysporum. This study provides a theoretical basis for the breeding of vegetable rootstock varieties with disease resistance and disease prevention and control.
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