Isolation, Identification and Degradation Characteristics of Highly Efficient Antibiotic Degrading Strains in Silkworm (Bombyx mori) Excrement
KONG Jia1, LI Hao1,2, JIANG Xue-Ping1, ZHOU Jie-Ling1, ZHANG Yuan-Hao1, CHEN Chen1, ZHANG Ran1,2, GUI Zhong-Zheng1,2,*
1 School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China; 2 Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Zhenjiang 212100, China
Abstract：Ciprofloxacin (CIP) and chloramphenicol (CAP), as commonly used veterinary antibiotics, persistently accumulate in the environment and pose significant ecological risks by potentially enhancing microbial resistance and threatening ecosystem and public health. In this study, a strain SMX capable of simultaneously degrading CIP and CAP from fermented silkworm (Bombyx mori) excrement was isolated, and identified as Phytobacter diazotrophicus through whole-genome sequencing. Then the process conditions for degrading antibiotics by strain SMX were optimized, the degradation efficiency of antibiotics was analyzed using high-performance liquid chromatography (HPLC), and the toxicity of the degradation solution to bioindicator bacteria was evaluated. Results showed that, the optimal conditions for strain SMX to degrade CIP were CIP initial concentration of 2.5 mg/L, 30 ℃, pH 8.0, and 3% inoculation amount, the optimal conditions for strain SMX to degrade CAP were CAP initial concentration of 10 mg/L, 30 ℃, pH 7.0, and 3% inoculation amount. HPLC analysis revealed that after being treated with strain SMX for 72 h, the degradation efficiencies of CIP and CAP were 87.37% and 86.32%, respectively. The degradation solution of strain SMX had almost no toxicity to bioindicator bacteria. These results showed that P. diazotrophicus strain SMX was an efficient and environmentally safe CIP/CAP degradation bacterium, which could play an active role in the prevention and control of antibiotic co-contamination. This study provides technical support for the biodegradation of antibiotic substances.
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