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Abstract Based on the sequence of RAPD marker OPS03-1354 linked to anthracnose (Sphaceloma ampelinum) resistance gene, two oligomers, R1 (5'-CAGAGGTCCCTAGCTAAT-3') and R2 (5'-ACAATCACCCAACTC CTC-3'), were designed and synthesized, and used as special primer for PCR analysis among the parents and 51 F1 individuals from interspecific cross combination Vitis pseudoreticulata Baihe-35-1×V. vinifera Carignane. A DNA band about 1 100 bp could be amplified among resistant plants using primer R2, and was present in resistant plants of 339 F1 individuals from interspecific cross combination Guangxi-1 (V.pseudoreticulata)×Jingkejing (V.vinifera) and of 47 lines (varieties) of grape resources, including Chinese wild Vitis, American wild Vitis and European grape, by PCR analysis. The result showed that the DNA band about 1 100 bp was a sequence-characterized amplified region (SCAR) marker linked to anthracnose-resistance gene in grape. The plants with the SCAR marker were the carrier and expression of anthracnose-resistance gene. Cloning and sequencing of the SCAR marker were carried out. The length of the SCAR marker was actually 1 110 bp and it was named SCS03-1110. This research reveals that the oligomer R2 (5'-ACAATCACCCAACTCCTC-3') as a DNA probe can be used to identify anthracnose-resistance gene of grape germplasm resources and hybrids from anthracnose-resistance breeding program by PCR assay.
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Received: 09 March 2009
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Corresponding Authors:
Yue-Jin Wang
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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