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| Cloning and Sequence Analysis of Mitochondrial Cytochrome b Gene(Cytb) in Mink(Mustela vison) and Its Evolutionary Relationships with the Mustela |
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Abstract A pair of primers were designed by referring to species close to mink(Mustela. vison), the complete mitochondrial cytochrome b gene(Cytb) sequences of six mink breeds and Zuojia mink were determined using cloning, sequenceing and assembling. Sequence analysis used DNAstar v6.13 and DnaSP4.0 revealed that Cytb Gene of minks were 1 140 bp in length, the composition of nucleotides A, G, T and C was 29.8%, 13.0%, 30.3%and 26.9%, respectively. Twelve variable sites were detected, and they all were transition. Multiple sequences alignments by ClustalX1.8 showed that they were high similarity in Cytb gene among minks. Combined with homologous sequences of 14 species in the Mustela retrieved from GenBank, the phylogenetic trees based on nucleotide and amino acid sequences were constructed by Neighbor-joining method, respectively. Two trees had same topologies. The results indicated that minks in China had closer evolutionary relationships with American minks than with European mink(M.lutreola). The divergence time between American mink and European mink might have started at Miocene.
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Received: 05 June 2009
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