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Genetic Analysis and SSR Location of Stripe Rust Resistance of Wheat Translocation Line M8657-1 Derived from Triticum aestivum-Leymus mollis (Trin.) Hara |
2, 2, 2, 2 |
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Abstract To identify and tag new resistance genes to stripe rust in a translocation line M8657-1 (commom wheat(Triticum aestivum) translocated with Leymus mollis (Trin.) Hara), the yellow rust races CYR30, CYR31, SU11-4 and SU11-11 were seleceted as inoculum. Genetic analysis of resistance to stripe rust was made in the progeny population of M8657-1/Mingxian169 at seedling stage. The results showed that one recessive gene conferred resistance to stripe rust race CYR30 and SU11-11, respectively; Two dominant karyoplasm genes conferred resistance to CYR31; And the resistance to SU11-4 was controlled by one dominant gene which was temporarily designated as YrElm1-4. The F2 population inoculated with SU11-4 were used for SSR analysis. SSR markers, as well as 320 pair primers, combined with bulked segregation analysis(BSA) revealed that three SSR markers,Xgwm636, Xwmc522 and Xwmc453, located on the short arm of chromosome 2A were linked to YrElm1-4. And YrElm1-4 was deduced to locate on the chromosome 2AS based on the three markers location. So the three SSR markers can be used in molecular-asisted breeding.
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Received: 29 June 2009
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