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Abstract Using plants bioreactor to produce pharmaceutical proteins has special development advantages, however, foreign gene silencing usually affecte gene expression. Since gene silencing suppressor can effectively inhibit the gene silencing，we focused on using gene silencing suppressor helper component-proteinase (HC-Pro) to improve gene expression. The results showed that green fluorescent protein (GFP) expressed in Nicotiana benthamiana plants by leaf infection of Agrobacterium suspension containing p35S-30B-GFP vector. Compared with the wild type tobacco Nb, the expression level of GFP significantly increased in HC-Pro transgenic tobacco analyzed by protein electrophoresis and Western blot. The results provide the basis for gene silencing suppressor applied in plant bioreactor.
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Received: 27 August 2013
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