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Expression, Chromosomal Location and Molecular Marker of High-Molecular-Weight Glutenin Subunit Gene of
Thinopyrum elongatum in Wheat Background |
| Tang Zhaohui Liu Shoubin You Mingshan Li Baoyun Mao Shanfeng Song Jianmin Liu Guangtian |
| (College of Agronomy and Biotechnology, China Agricultural University , Beijing 100094, China) |
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Abstract Abstract：High-molecular-weight glutenin subunits (HMW-GS) are main factors that affect bread-making quality of common wheat. Many common wheat cultivars derived from the Thinopyrum germplasm have good bread-making quality. But HMW-GS coded by E-genome of Thinopyrum was not reported previously. In the paper, the HMW-GS compositions of Thinopyrum elongatum (2n=14, EE) were analyzed by SDS-PAGE technique with materials of wheat (Triticum aestivum) var. Chinese Spring (CS), Thinopyrum elongatum (PI547326), CS-Th. elongatum amphidiploids, alien disomic and ditelosomic addition (DA) and alien disomic substitution (DS) lines. Studies on chromosomal location of HMW-GS gene of E-genome was performed in the meantime, and works of amplifying the HMW-GS gene of E-genome was also conducted by using PCR primers specific for the repetitive region of y-type HMW-GS genes. The main results were showed bellow:
1. E-genome in CS background coded one single HMW-GS with a highly similar migration ratio to subunit 1By8 of CS in SDS-PAGE gel, and in the accession of Thinopyrum elongatum PI547326 coded one subunit similar to 1By9 of Chenny. These two subunits were therefore temporarily named 1E8 and 1E9 respectively. 2. The HMW-GS gene of E-genome was located in the long arm of chromosome 1E. This result was consistent with the fact that the arrangement of HMW-GS genes was collinear in chromosomes of grain crops. 3. With primers for HMW-GS genes, one specific fragment in the approximately length of 1 300 bp was amplified in all materials containing 1EL chromosome. This fragment could be used as molecular marker for the HMW-GS gene of E-genome.
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Received: 01 January 1900
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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