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Molecular Cloning and Expression of EIN3 (Ethylene Insensitive 3)
Gene in Tomato Fruit |
| Xu Jingyu Luo Yunbo** Wei Shaochong |
| (College of Food Science, China Agricultural University, Beijing 100094, China) |
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Abstract Abstract: EIN3(ethylene insensitive 3) in Arabidopsis thaliana is a novel nuclear-localized protein, which is thought to serve as a transcription factor in response to ethylene signal. The cDNA of homologous genes of A.thaliana EIN3 was obtained from tomato (Lycopersicon esculentum ) fruit by RT-PCR amplification and cDNA library screening，termed Le-EIL . The cDNA clone contained an open reading frame of 1 845 bp , encoding a sequence of 615 amino acid. Primary sequence analysis indicated that it exhibited 79% sequence similarity to tobacco(Nicotiana tabacum ?雪 TEIL and the sequence similarity of this clone to A.thaliana EIN3, EIL1,EIL2 and EIL3 was 59%、56%、52%、and 46% respectively. Its expression patterns in both wild type tomato and antisense-ACS transgenic tomato fruit development showed that the expression of Le-EIL gene highly relatived to ripening and ethylene synthesis. In wild type tomato fruit, the expression of Le-EIL gene increased as the fruits got ripening, and reached its peak level at turning and pink stage, then decreased. In antisense-ACS transgenic tomato fruit, the expression of Le-EIL was weak at 60 d fruit past anthesis but disappeared at the other ripening stages.
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Received: 01 January 1900
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