Abstract Primordial germ cells (PGCs) were isolated from the gonads of chicken embryos at stage 28, PGCs were co-cultured with gonadal stroma cells. Identification of PGCs was carried out by periodic acid-Schiff (PAS) and alkaline phosphatase (AKP) staining. We constructed a lentiviral vector pLenti-CMV-eGFP and harvested the virus by cotransfecting 293FT cells with the vector and packaging plasmids. Lentiviruses after concentration were used to transfect chicken PGCs, the transfection efficiency of PGCs was up to 24.19%. The impacts of different volume of lentiviruses on transfection efficiencies were also compared, the results indicated that as the increase of lentiviruses, the transfection efficiency was significantly improved.
