|
|
|
| Cloning of ACO Gene and Inhibition of Ethylene Production with RNAi in tomato |
| |
| |
|
|
|
|
Abstract A fragment of 1018 bp of ACO gene cDNA sequence was cloned using RT-PCR with two PCR primers designed according to the sequence of tomato cDNA clone (E11). The result of BLAST showed the sequence presented a very high match with the ACO genes from other plants; the homologue is from 83% to 99%. Using the sequence, an RNA interference (RNAi) transformation vector (pD311) was constructed through the way of BP cloning. The transformation was performed to tomato. 27 regenerated plants with kanamycin resistance were obtained. And the transgene integrated into tomato genome was proved with PCR and Southern blotting. The ethylene respiration amount of the RNAi transgenic tomato plants was measured by gas chromatography. The results showed that ethylene evolution was specifically inhibited in the leaves and fruits of transgenic plants.
|
|
Received: 21 August 2006
|
|
|
|
|
|
|
