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| Studies on Improving T-A Cloning Efficiency of the PCR Products |
| Li Ruiguo An Xiaorong Gou Kemian Bo Jialin Hou Jian Chen Yongfu** |
| (State Key Laboratoy for Agrobiotechnology, China Agricultural University, Beijing 100094, China) |
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Abstract Abstract:Three kinds of T-A cloning methods were tested to compare the efficiency of four different length PCR products. The results indicated that high cloning efficiency was obtained using common T-A cloning method for short PCR product(Length is about 500 bp), the cloning efficiency was improved using temperature cycle T-A cloning method for moderate PCR product(Length is about 3 000 bp) and the cloning efficiency was increased using two-step recombinant T-A cloning method for long PCR product(Length is about 6 000 bp).
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Received: 01 January 1900
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