Contact Us Add to Favorite
 
NianQi Search Adv Search
33
研究论文 Current Issue | Archive | Adv Search |
Studies on Improving T-A Cloning Efficiency of the PCR Products
Li Ruiguo An Xiaorong Gou Kemian Bo Jialin Hou Jian Chen Yongfu**
(State Key Laboratoy for Agrobiotechnology, China Agricultural University, Beijing 100094, China)
Download: PDF (250 KB)   HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      
Abstract  Abstract:Three kinds of T-A cloning methods were tested to compare the efficiency of four different length PCR products. The results indicated that high cloning efficiency was obtained using common T-A cloning method for short PCR product(Length is about 500 bp), the cloning efficiency was improved using temperature cycle T-A cloning method for moderate PCR product(Length is about 3 000 bp) and the cloning efficiency was increased using two-step recombinant T-A cloning method for long PCR product(Length is about 6 000 bp).
Key wordsKey words:T-A cloning      PCR products      cloning efficiency     
Received: 01 January 1900     
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Li Ruiguo An Xiaorong Gou Kemian Bo Jialin Hou Jian Chen Yongfu**
Cite this article:   
Li Ruiguo An Xiaorong Gou Kemian Bo Jialin Hou Jian Chen Yongfu**. Studies on Improving T-A Cloning Efficiency of the PCR Products [J]. , 2003, 11(2): 158-162.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/      OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2003/V11/I2/158
Copyright © Editorial Board of 农业生物技术学报
Supported by:Beijing Magtech