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| Construction of a New Plant Transformation Vector pBG1112 with a Chitinase Gene SchiA from Serratia marcescens and Its Genetic Transformation in Rice |
| He Yingchun1 Li Xiaoxiang2 Gao Bida1 |
(1. College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Rice Research Institute, Changsha 410125, China) |
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Abstract Abstract: A 2.8 kb CaMV 35S promoter/Schi A coding region/Nos terminator fusion gene was inserted into the polycloning site of a 11.8 kb binary vector pCAMBIA1301 to produce a new 14.6 kb plasmid pBG1112, which was used in rice (Oryza sativa L.) transformation by floral organ-mediated method. T3 rice plants were screened by hygromycin solution and the positive plants were examined by PCR for presence of transgene and by RT-PCR for matured mRNA. Bioassay for some of the T3 transgenic rice plants which displayed both hygromycin-resistance and RT-PCR positive showed the increased resistance to rice sheath blight (Rhizoctonia solani ) and rice blast（Pyricularia oryzae）. The sequencing result showed that the nucleotide acid sequences of RT-PCR products were the same as that of transgene analyzed by BLAST software. Chitinase activity of T4 transgenic rice was higher than that of non-transgenic rice,which showed that the transferred exogenous chitinase gene could be expressed normally.
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Received: 01 January 1900
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