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Construction of a New Plant Transformation Vector pBG1112 with a Chitinase Gene SchiA from Serratia marcescens  and Its Genetic Transformation in Rice
He Yingchun1 Li Xiaoxiang2 Gao Bida1
(1. College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Rice Research Institute, Changsha 410125, China)
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Abstract  Abstract: A 2.8 kb CaMV 35S promoter/Schi A coding region/Nos terminator fusion gene was inserted into the polycloning site of a 11.8 kb binary vector pCAMBIA1301 to produce a new 14.6 kb plasmid pBG1112, which was used in rice (Oryza sativa L.) transformation by floral organ-mediated method. T3 rice plants were screened by hygromycin solution and the positive plants were examined by PCR for presence of transgene and by RT-PCR for matured mRNA. Bioassay for some of the T3 transgenic rice plants which displayed both hygromycin-resistance and RT-PCR positive showed the increased resistance to rice sheath blight (Rhizoctonia solani ) and rice blast(Pyricularia oryzae). The sequencing result showed that the nucleotide acid sequences of RT-PCR products were the same as  that of transgene analyzed by BLAST software. Chitinase activity of T4 transgenic rice was higher than that of non-transgenic rice,which showed that the transferred exogenous chitinase gene could be expressed normally. 
Key wordsKey words: bacterial chitinase gene      transformation      rice      rice sheath blight, rice blast      
Received: 01 January 1900     
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He Yingchun
Li Xiaoxiang
Gao Bida
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He Yingchun,Li Xiaoxiang,Gao Bida. Construction of a New Plant Transformation Vector pBG1112 with a Chitinase Gene SchiA from Serratia marcescens  and Its Genetic Transformation in Rice[J]. , 2003, 11(2): 121-126.
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http://journal05.magtech.org.cn/Jwk_ny/EN/      OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2003/V11/I2/121
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