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| A Method of Designing Tail Sequence for Maize SSR Primers |
| Meng KUANG Jiu-Ran ZHAO |
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Abstract Tail sequence is a primer sequence which is not belong to maize genome and can be widely applied in maize SSR primers. A lot of common maize SSR primers can be used in fluorescence detection system just by labeling 5` end of the tail sequence with fluorescence, then high-through and low cost detection could be achieved at the same time. A method of designing tail sequence was discussed, including design principle, design process, universality and PCR conditions.
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Received: 04 January 2007
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Corresponding Authors:
Jiu-Ran ZHAO
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