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Abstract Lenok (Brachymystax lenok), which was a famous endemic fish in China, had undergone a dramatic decline for the impacts of human and environment. In this paper, the genetic diversity of the seventy-two individuals, which from the three wild populations of Mudanjiang River (MD), Yalujiang River (YL) and Wusuli River (WSL) respectively, were analyzed using the AFLP method. In total, 541 polymorphic loci of 559 were amplified with 12 primer pairs and the percentage of polymorphic loci was 96.78 %. The Shannon information index of the MD, YL and WSL populations was 0.3988±0.2913, 0.3254±0.3037, 0.2125±0.2862, respectively. And the Nei's gene diversity index was 0.2737±0.2062, 0.2229±0.2129, 0.1446±0.1985, respectively. The average Ht was 0.3512±0.0.0208 and Hs was 0.2137±0.0152. Among the three populations the average genetic distance (Dst) was 0.1375. The gene differentiation coefficient (Gst) of 0.3914 indicated that there was genetic differentiation of a certain degree among the populations. The genetic diversity was 60.85% within population and 39.15% among the population. The gene flow index (Nm) was 0.7776. The AMOVA analysis indicated that the average Fst was 0.55336. 44.84% of variance was among populations and 55.16% within populations. The MD group showed the highest polymorphism ratio and the lowest in WSL group.
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Received: 14 July 2008
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