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Abstract Using the polyclonal antiserum against Spodoptera exigua (Hübner) invertebrate intestinal mucin (IIM), a solute carrier gene LstiSLC25 was screened from the cDNA expression library of Loxostege sticticalis midgut. The full-length of LstiSLC25 is 1399bp (GenBank accession no. EU924506) and the open reading frame(ORF) was 897bp, encoding 299 amino acid residues; the predicted MW and pI were 32.1kD and 9.46, respectively. The LstiSLC25 possesses three solcars, which is characterized of solute carrier family. The LstiSLC25 gene has been recombined into pET30 vector. The Western blot analysis demonstrated that LstiSLC25 protein was expressed at low level after being induced by IPTG.
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Received: 09 September 2008
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