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Abstract Based on the gene sequence of cap of PCV2, a pair of primers was designed and, a 384 bp fraction of the carboxyl end of the capsid protein gene was obtained by PCR amplification, which was then cloned into plasmid pR to yield an integrative plasmid pR-cap. The integrative recombinant plasmid was used to transform E. coli E2 host bacteria, and then this E2 containing the recombinant plasmid was used for homologous recombination with lysozyme gene-deficient T4, thus yielding a recombinant bacteriophage T4-cap. When purified T4-CAP was tested with SDS-PAGE and Western blotting, a 25 Ku protein band was detected in polyacrylamide gel and nitrocelluLose membrane, which could combine specifically with PCV2 antiserum, attesting to the successful display of SOC fusion protein on the T4 bacteriophage surface.
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Received: 06 July 2007
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