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| Study on Purification and Antibacterial Activity of Recombinant Human Lactoferrin in Milk of Transgenic Mice |
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Abstract In this paper, to confirm the bacteriostatic properties， the recombinant human lactoferrin (rhLF) was extracted form the milk of transgenic mice by gel filtration chromatography. The rhLF was also analyzed by SDS-PAGE and ELISA-assay. The bacteriostatic properties of rhLF was tested by agar disc diffusion method and direct counting method. The results showed that the molecular weights of rhLF, both of proteins from the transgenic mice of PCL25 and AP vectors were compared with that of native hLF, and were about 78 KDa. The rhLFs have significant bacteriostatic properties against E.coli and Samonella.
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Received: 04 August 2008
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