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Abstract The objective of this study was to examine the possibility of using a non-electrophoretic method to detect the TSPY gene for sexing bovine early embryos, which made bovine embryo sexing under farm condition more feasible. Primers were designed to amplify a portion of the TSPY gene and a common gene as an internal control primer. PCR optimization was carried out using a DNA template from bovine whole blood. Furthermore, six embryo samples were diagnosed by this method and the sexing results were contrasted with those of the Loop-Mediated Isothermal Amplification (LAMP) method. The result showed that TSPY was as reliable a sexing method as LAMP. Forty-three morula and blastocyst embryos collected from superovulated donor dairy cattle were sexed by this method, and twenty-one embryos judged to be female embryos were transferred non-surgically to recipients 6 to 8 days after natural estrus. Out of 21 recipients, 9 were pregnant and all delivered female calves. The results showed that the sex predicted accuracy of this protocol was 100%. In conclusion, TSPY gene was a good male specific marker and indicated that a non-electrophoretic method was feasible and accurate to detect TSPY gene for sexing preimplantation bovine embryos.
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Received: 23 May 2008
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NI He-Jia, CHENG Xue, WANG Jing, GAO Ze-Ping, WEI Pan-Pan, TAN Jia-Li, SUN Jing, LI Hai-Tao, GAO Ji-Guo. Isolation and Screening of High Virulent Bacillus thuringiensis Strains Against Lepidopteran Pests in Some Areas of Liaoning Province[J]. 农业生物技术学报, 2019, 27(8): 1513-1520. |
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