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Abstract R2R3-MYB is a principal member of MYB transcription factor superfamily, which has been showed to play an important role in secondary metabolism and abiotic stress responses. In order to obtain a full-length sequence of R2R3-MYB transcription factor gene and its expression profile under different abiotic stresses, a full-length cDNA sequence of sugarcane(Saccharum complex) R2R3-MYB-like gene named as Sc2RMyb1 (GenBank Accession No. JQ823165) was cloned by combined sugarcane EST databases, electronic cloing technology and PCR amplification. Genomic DNA sequence of Sc2RMyb1 was 1 807 bp in length, including 3 exons and 2 introns, and the complete coding sequence was 1 248 bp encoding 427 amino acids. The recombinant protein with an estimated molecular mass of 52 kD was produced in positive prokaryotic strain induced by IPTG. The recombinant Escherichia coli cells exhibited better growth than that of the negative strain in liquid LB medium with addition of NaCl. When 25rRNA gene was used as the internal control, the expression profiles of Sc2RMyb1 gene responsive to different stresses were detected by Real-time qPCR in sugarcane tissue culture seedling. The transcript levels of Sc2RMyb1 gene in sugarcane seedling reduced under H2O2 and NaCl stress, respectively. It was suggested that the Sc2RMyb1 gene, as a negative adjustment factor, might involve in the response to salt stress. The results of this study provide basic information for the further research of the Sc2RMyb1 gene in sugarcane stress tolerance mechanisms and resistance breeding
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Received: 27 December 2011
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