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| Cloning and Correlation Analysis of PsPⅡ Gene Associated with the Release of Dormant Buds in Tree Peony(Paeonia suffruticosa) |
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Abstract In order to investigate the relationship between PsPⅡ gene and dormancy release by low temperature, using tree peony(Paeonia suffruticosa) 'Lu He Hong' as plant materials, the full length of PsPⅡ, bioinformatics analysis and expression pattern were conducted under different chilling treatments. The main results were as follows: The full-length cDNA was 899 bp, containing an open reading frame of 603 bp, which encoded a 200 amino acid polypeptide. Its 5' untranslated regions was 70 bp, 3' UTR was 226 bp, including a 24 bp poly(A) tail. It was predicted that PⅡ protein of tree peony was non-secretory hydrophilic protein, modulating the activity of glutamine synthetase. Homology and phylogenetic relationships analysis revealed that PⅡ protein in rice was on the same branch with PⅡ protein in tree peony and there was an identity of 83.6% at amino acid homology. The following species were Nicotiana tabacum, Arabidopsis Thaliana and Pinus pinaster, respectively. By Real-time PCR, we demonstrated that the expression of PsPⅡ was enhanced and continuously increased in the buds of tree peony during the release of dormancy by chilling treatment, reaching the highest value during 15 days chilling treatment. Significant positive correlation between the expression level of PsPⅡ and α-starch hydrolytic enzymes, the activity of glutamine synthase and soluble carbohydrate was observed. This result indicted that the role of PsPⅡ was to increase the activity of enzymes associated with nutrient metabolism during the process of dormancy release.
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Received: 21 July 2012
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