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| cDNA Library Construction of Salicornia bigelovii Torr. Under Salt Stresses and Analysis of Tolerance Related Gene |
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Abstract Salicornia bigelovii Torr. is one of the most salt-tolerant plant species in the world. In order to study the mechanism of salt tolerance and isolate new genes related to salt tolerance in S. bigelovii Torr., a normalized full-length cDNA library in S. bigelovii Torr. was constructed by SMART TM(switching mechanism at 5’end of RNA transcript) technique. The primary library had a titer of 5.46×105 pfu/mL, and it’s recombination rate reached 96.7%. The average size range of cDNA inserts was 0.5~2 kb, 60％ of them more than 1.0 kb. 100 clones were randomly selected and sequenced, and then preliminary analysis was performed using bioinformatics software, 65 gene sequences were generated from the cDNA library. By Blast searches, we found that 35 genes showed significant similarity to the annotated genes, and the remaining 30 genes represented novel genes without any annotation. In addition, we analyzed SbX and SbSAMS mRNA level by semi-quantity RT-PCR in S. bigelovii Torr. under 400 mmol/L NaCl. We found that SbX was up-regulated by salt stress, and the expression of SbSAMS was opposite. The results may provide basic information about the mechanism of salt tolerance in plants, improvement and germplasm enhancement of crop genetic traits.
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Received: 08 October 2012
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