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Abstract slc30A family(vertebrate cation diffusion facilitator family proteins) transport zinc from the cytoplasm to the lumen, thus decreasing the content of zinc in cytosol. Cloning primers were designed according to the cloning principle of homologous sequence in this paper, the slc30A1~slc30A9 genes were cloned by RT-PCR, then compared cloning sequences of slc30A1~slc30A9 and protein amino acid coding by these gene sequences with other species for homology analysis, and we also studied the slc30A1~slc30A9 distribution in the pig tissue. The main results showed that: The sequence length of gene slc30A1~slc30A9 was about 1 044~2 301 bp all with a complete open reading frame (ORF), which medicated to encode 348~767 amino acid residues, respectively(GenBank accession No. FJ374262.1, EU825192.2, FJ358706.1, EU835903.1, FJ230771.1, FJ237622.1, FJ237623.1, FJ588029 and FJ164069.1, respectively), but slc30A8 was the only mutant. The homology analysis results of slc30A and ZnT(zinc transporter) protein amino acid nearly were the same, higher with human(Homo taurus) and cow(Bos taurus), but lower with Rat(Rattus nurvegicus) and Mouse(Mus musculus), and difference were present in the different isomers and its coding proteins. The results of tissue distribution suggested that slc30A8 was only found in the ileum, slc30A9 was found in the esophagus and all tract, while slc30A1 and slc30A6 were found in other tissue except in the ileum. The other isomers were not found in the ileum, but there were different degree distribution in other tissue. These results suggested that the difference of the slc30A1~ slc30A9 genes distribution in all tissues maybe related to its special function in pig tissue
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Received: 27 March 2012
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