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| Construction of DH Population from Indica-Japonica Cross and Analysis of Distorted Segregation in Genotype |
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Abstract The project was to distinguish accurately homozygous regenerants using molecular markers in early stages of rice anther culture and to analysize distroted segregation in genotype of the population , to constuct DH population quickly and exactly. Hybrid of japonica/indica combination Zhonghua 11/Zhongzu 14 was constructed and anther culture of the hybrid was carried out. 46 polymorphic markers from 228 ones between the two parents had been selected. All of 112 regenerants were distinguished homozygous using 22 of the 46 polymorphic markers. It suggested that the regenerants population was accuratedly obtained from microspore. The 22 markers were used to analysize distorted segregation of the obtained population in genotype. Results showed that it was in continuous segregation and showed normal distribution. The project indicated the DH population was constructed accurately with microspore-derived regenerants and normal sgregation using SSR polymorphic markers, and which could be efficiently carried out earlier using SSR markers.
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Received: 26 August 2007
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