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| Cloning of full length cDNA of triose phosphate translocator from corn and its expression in transgenic tobacco |
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Abstract The triose phosphate translocator(TPT)cDNA of full length was amplified by RT-PCR. Sequence analysis indicated that the full length cDNA fragment was showing 99.9％ homology to the sequence reported before．Only one base is different．The sense expression vector with TPT full length cDNA under
the control of CaMV 35S promoter was constructed and were transformed into tobacco by leaf disc transformation．The transformed tobaccos were identified by PCR and Southern blotting analysis．
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Received: 18 June 2007
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