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| Cloning and sequence analysis of RAPD markers linked to the resistant powdery mildew genes in Chinee wild Vitis |
| Zhang Jian-xia Wang Yue-jin Zhou Bang-jun Xu Wei-rong |
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Abstract OPW02-1756, OPO11-964, OPY13-661, OPB11-520, OPW05-766, OPV03-1365 and OPJ16-759, seven RAPD markers linked to the resistant powdery mildew genes in Chinese wild Vitis were reclaimed, cloned, sequenced and analyzed. The results showed that the real length of seven RAPD markers was 1756bp, 964bp, 661bp, 520bp, 766bp, 1365bp and 759bp respectively. Marker OPW02-1756 shows 94% to 98% identity with 3 EST sequences from European grapes. OPO11-964 shows 93 % identity with 1 EST sequence from Cabernet Sauvignon, shows 90% and 87% identity respectively with 2 EST sequences from green grape berries library, shows 83% identity with 1 EST sequence from multiple pathogen-infected sweet orange. OPO11-964 sequence has a biggest open reading frame of 444bp encoding 147 amino acids, has partial identity with 21 protein sequences of unknown functions from other lives. OPY13-661 sequence has identity with 16 EST sequences from European grapes, and shows 89% identity with 2 EST sequences from abiotic stressed leaves of Vitis vinifera var. Chardonnay. OPB11-520 shows 94% identity with 4 genomic sequences of unknown functions of Arabidopsis thaliana. OPW05-766 shows 50% identity with GAG-POL precursor of wine grape. OPV03-1365 shows 81% to 88% identity with 6 rice genomic sequences, 84% to 91% identity with 3 sequences from Arabidopsis thaliana. OPJ16-759 shows 83% to 100% identity with 14 EST sequences from abiotic stressed leaves of Vitis vinifera var. Chardonnay, 94% to 100% identity with 3 EST sequences from water stressed berry of Vitis vinifera var. Cabsau.
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Received: 29 October 2007
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Corresponding Authors:
Zhang Jian-xia
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